Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/8924
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Type: Journal article
Title: Prodomain-dependent neclear localisation of the caspase-2 (Nedd2) precursor: A novel fuction for a caspase prodomain
Author: Colussi, P.
Harvey, N.
Kumar, S.
Citation: Journal of Biological Chemistry, 1998; 273(38):24535-24542
Publisher: American Society for Biochemistry and Molecular Biology
Issue Date: 1998
ISSN: 1083-351X
1083-351X
Statement of
Responsibility: 
Paul A. Colussi, Natasha L. Harvey and Sharad Kumar
Abstract: Caspases are cysteine proteases that play an essential role in apoptosis by cleaving several key cellular proteins. Despite their function in apoptosis, little is known about where in the cell they are localized and whether they are translocated to specific cellular compartments upon activation. In the present paper, usingAequorea victoria green fluorescent protein fusion constructs, we have determined the localization of Nedd2 (mouse caspase-2) and show that both precursor and processed caspase-2 localize to the cytoplasmic and the nuclear compartments. We demonstrate that the nuclear localization of caspase-2 is strictly dependent on the presence of the prodomain. A caspase-2 prodomain-green fluorescent protein localized to dot- and fiber-like structures mostly in the nucleus, whereas a protein lacking the prodomain was largely concentrated in the cytoplasm. We also show that an amino-terminal fusion of the prodomain of caspase-2 to caspase-3 mediates nuclear transport of caspase-3, which is normally localized in the cytoplasm. These results suggest that, in addition to roles in dimerization and recruitment through adaptors, the caspase-2 prodomain has a novel function in nuclear transport.
Keywords: COS Cells
3T3 Cells
Cell Nucleus
Cytoplasm
Animals
Mice
Scyphozoa
Enzyme Precursors
Cysteine Endopeptidases
Caspases
Proteins
Luminescent Proteins
Green Fluorescent Proteins
Recombinant Fusion Proteins
DNA Primers
Transfection
Protein Biosynthesis
Caspase 2
Rights: © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
DOI: 10.1074/jbc.273.38.24535
Published version: http://dx.doi.org/10.1074/jbc.273.38.24535
Appears in Collections:Aurora harvest
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