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Type: Journal article
Title: Characterization of bone marrow derived mesenchymal stem cells in suspension
Author: Akiyama, K.
You, Y.
Yamaza, T.
Chen, C.
Tang, L.
Jin, Y.
Chen, X.
Gronthos, S.
Shi, S.
Citation: Stem Cell Research and Therapy, 2012; 3(5):40-1-40-13
Publisher: BioMed Central
Issue Date: 2012
ISSN: 1757-6512
Statement of
Kentaro Akiyama, Yong-Ouk You, Takayoshi Yamaza, Chider Chen, Liang Tang, Yan Jin, Xiao-Dong Chen, Stan Gronthos, and Songtao Shi
Abstract: INTRODUCTION: Bone marrow mesenchymal stem cells (BMMSCs) are a heterogeneous population of postnatal precursor cells with the capacity of adhering to culture dishes generating colony-forming unit-fibroblasts (CFU-F). Here we identify a new subset of BMMSCs that fail to adhere to plastic culture dishes and remain in culture suspension (S-BMMSCs). METHODS: To catch S-BMMSCs, we used BMMSCs-produced extracellular cell matrix (ECM)-coated dishes. Isolated S-BMMSCs were analyzed by in vitro stem cell analysis approaches, including flow cytometry, inductive multiple differentiation, western blot and in vivo implantation to assess the bone regeneration ability of S-BMMSCs. Furthermore, we performed systemic S-BMMSCs transplantation to treat systemic lupus erythematosus (SLE)-like MRL/lpr mice. RESULTS: S-BMMSCs are capable of adhering to ECM-coated dishes and showing mesenchymal stem cell characteristics with distinction from hematopoietic cells as evidenced by co-expression of CD73 or Oct-4 with CD34, forming a single colony cluster on ECM, and failure to differentiate into hematopoietic cell lineage. Moreover, we found that culture-expanded S-BMMSCs exhibited significantly increased immunomodulatory capacities in vitro and an efficacious treatment for SLE-like MRL/lpr mice by rebalancing regulatory T cells (Tregs) and T helper 17 cells (Th17) through high NO production. CONCLUSIONS: These data suggest that it is feasible to improve immunotherapy by identifying a new subset BMMSCs.
Keywords: Bone Marrow Cells; Stem Cells; Animals; Mice, Inbred C3H; Mice, Inbred C57BL; Humans; Mice; Mice, Nude; Cell Proliferation; Female; Mesenchymal Stromal Cells
Rights: © 2012 Akiyama et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0030017537
DOI: 10.1186/scrt131
Appears in Collections:Medical Sciences publications

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