Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/89264
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Type: Journal article
Title: Characterization of bone marrow derived mesenchymal stem cells in suspension
Author: Akiyama, K.
You, Y.
Yamaza, T.
Chen, C.
Tang, L.
Jin, Y.
Chen, X.
Gronthos, S.
Shi, S.
Citation: Stem Cell Research and Therapy, 2012; 3(5):40-1-40-13
Publisher: BioMed Central
Issue Date: 2012
ISSN: 1757-6512
1757-6512
Statement of
Responsibility: 
Kentaro Akiyama, Yong-Ouk You, Takayoshi Yamaza, Chider Chen, Liang Tang, Yan Jin, Xiao-Dong Chen, Stan Gronthos, and Songtao Shi
Abstract: INTRODUCTION: Bone marrow mesenchymal stem cells (BMMSCs) are a heterogeneous population of postnatal precursor cells with the capacity of adhering to culture dishes generating colony-forming unit-fibroblasts (CFU-F). Here we identify a new subset of BMMSCs that fail to adhere to plastic culture dishes and remain in culture suspension (S-BMMSCs). METHODS: To catch S-BMMSCs, we used BMMSCs-produced extracellular cell matrix (ECM)-coated dishes. Isolated S-BMMSCs were analyzed by in vitro stem cell analysis approaches, including flow cytometry, inductive multiple differentiation, western blot and in vivo implantation to assess the bone regeneration ability of S-BMMSCs. Furthermore, we performed systemic S-BMMSCs transplantation to treat systemic lupus erythematosus (SLE)-like MRL/lpr mice. RESULTS: S-BMMSCs are capable of adhering to ECM-coated dishes and showing mesenchymal stem cell characteristics with distinction from hematopoietic cells as evidenced by co-expression of CD73 or Oct-4 with CD34, forming a single colony cluster on ECM, and failure to differentiate into hematopoietic cell lineage. Moreover, we found that culture-expanded S-BMMSCs exhibited significantly increased immunomodulatory capacities in vitro and an efficacious treatment for SLE-like MRL/lpr mice by rebalancing regulatory T cells (Tregs) and T helper 17 cells (Th17) through high NO production. CONCLUSIONS: These data suggest that it is feasible to improve immunotherapy by identifying a new subset BMMSCs.
Keywords: Bone Marrow Cells; Stem Cells; Animals; Mice, Inbred C3H; Mice, Inbred C57BL; Humans; Mice; Mice, Nude; Cell Proliferation; Female; Mesenchymal Stromal Cells
Rights: © 2012 Akiyama et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0030017537
DOI: 10.1186/scrt131
Appears in Collections:Medical Sciences publications

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