Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/8983
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dc.contributor.authorZannettino, A.en
dc.contributor.authorRayner, J.en
dc.contributor.authorAshman, L.en
dc.contributor.authorGonda, T.en
dc.contributor.authorSimmons, P.en
dc.date.issued1996en
dc.identifier.citationJournal of Immunology, 1996; 156(2):611-620en
dc.identifier.issn0022-1767en
dc.identifier.issn1550-6606en
dc.identifier.urihttp://hdl.handle.net/2440/8983-
dc.description.abstractcDNA expression cloning using retroviral vectors provides a means of stably introducing genes into target cells at efficiencies that surpass those achieved by transfection. Furthermore, retroviral vectors allow for the introduction and expression of complex cDNA libraries in a wide range of cell types, including cells of hemopoietic origin. Here we report a novel method for rapidly isolating genes encoding cell surface molecules (CSM) from a human bone marrow stromal cell cDNA library constructed in the retroviral vector, pRUFneo. With a newly described, highly efficient selection strategy using mAb and Ab-coated magnetic beads, we have successfully isolated six cDNA encoding previously defined CSM, including beta 1 integrin and endoglin. Moreover, we have used this approach to define the gene and hence the CSM identified by three previously unclustered mAb. These results confirm previous studies demonstrating the general utility of retroviral cDNA libraries and further extend their use to the expression cloning of cDNA encoding CSM.en
dc.description.statementofresponsibilityAndrew C. W. Zannettino, John R. Rayner, Leonie K. Ashman, Thomas J. Gonda and Paul J. Simmonsen
dc.language.isoenen
dc.publisherAMER ASSOC IMMUNOLOGISTSen
dc.rights© 1996 by The American Association of Immunologistsen
dc.source.urihttp://www.jimmunol.org/content/156/2/611en
dc.subjectConnective Tissue; Bone Marrow Cells; Hematopoietic Stem Cells; Cell Line; Animals; Humans; Mice; Retroviridae; Membrane Proteins; Recombinant Fusion Proteins; DNA, Complementary; Antibodies, Monoclonal; Antigens, Surface; Immunomagnetic Separation; Cloning, Molecular; Polymerase Chain Reaction; Gene Expression; Base Sequence; Gene Library; Genes; Genetic Vectors; Molecular Sequence Data; Adulten
dc.titleA powerful new technique for isolating genes encoding cell surface antigens using retroviral expression cloningen
dc.typeJournal articleen
dc.identifier.rmid0030004859en
dc.identifier.pubid68865-
pubs.library.collectionMedicine publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidZannettino, A. [0000-0002-6646-6167]en
dc.identifier.orcidGonda, T. [0000-0002-8792-3021]en
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