Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/90074
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Type: Journal article
Title: Drosophila integrin-linked kinase is required at sites of integrin adhesion to link the cytoskeleton to the plasma membrane
Author: Zervas, C.
Gregory, S.
Brown, N.
Citation: The Journal of Cell Biology, 2001; 152(5):1007-1018
Publisher: Rockefeller University Press
Issue Date: 2001
ISSN: 0021-9525
1540-8140
Statement of
Responsibility: 
Christos G. Zervas, Stephen L. Gregory, and Nicholas H. Brown
Abstract: Integrin-linked kinase (ILK) was identified by its interaction with the cytoplasmic tail of human β1 integrin and previous data suggest that ILK is a component of diverse signaling pathways, including integrin, Wnt, and protein kinase B. Here we show that the absence of ILK function in Drosophila causes defects similar to loss of integrin adhesion, but not similar to loss of these signaling pathways. ILK mutations cause embryonic lethality and defects in muscle attachment, and clones of cells lacking ILK in the adult wing fail to adhere, forming wing blisters. Consistent with this, an ILK–green fluorescent protein fusion protein colocalizes with the position-specific integrins at sites of integrin function: muscle attachment sites and the basal junctions of the wing epithelium. Surprisingly, mutations in the kinase domain shown to inactivate the kinase activity of human ILK do not show any phenotype in Drosophila, suggesting a kinase-independent function for ILK. The muscle detachment in ILK mutants is associated with detachment of the actin filaments from the muscle ends, unlike integrin mutants, in which the primary defect is detachment of the plasma membrane from the extracellular matrix. Our data suggest that ILK is a component of the structure linking the cytoskeleton and the plasma membrane at sites of integrin-mediated adhesion.
Keywords: integrins; cell adhesion; cytoskeleton; kinase; Drosophila
Rights: © The Rockefeller University Press
RMID: 0030013035
DOI: 10.1083/jcb.152.5.1007
Appears in Collections:Genetics publications

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