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https://hdl.handle.net/2440/90085
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Type: | Book chapter |
Title: | PPAR-alpha cloning, expression, and characterization |
Author: | Ngo, S.N. McKinnon, R.A. |
Citation: | Peroxisome Proliferator-Activated Receptors (PPARs): methods and protocols, 2013 / Badr, M.Z., Youssef, J.A. (ed./s), vol.952, Ch.2, pp.7-34 |
Publisher: | Humana Press |
Issue Date: | 2013 |
Series/Report no.: | Methods in Molecular Biology; 952 |
ISBN: | 9781627031554 |
Editor: | Badr, M.Z. Youssef, J.A. |
Statement of Responsibility: | Suong N. T. Ngo and Ross A. McKinnon |
Abstract: | Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays an important role in the regulation of many genes involved in key metabolic processes. Today, PPARα has been cloned from mammalian, marsupial, and a number of marine species and its expression has been found to be relatively tissue- and species-specific. Here, we describe the methods for cloning of PPARα genes by RT-PCR and RACE approaches and related protocols for studying the expression of cloned PPARα cDNAs in mammalian cell systems. |
Keywords: | PPARα cloning RT-PCR RACE Immunoblots Cos-7 cell expression system |
Rights: | © Springer Science+Business Media New York 2013 |
DOI: | 10.1007/978-1-62703-155-4_2 |
Published version: | http://dx.doi.org/10.1007/978-1-62703-155-4_2 |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest 2 |
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