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Type: Journal article
Title: HvZIP7 mediates zinc accumulation in barley (Hordeum vulgare) at moderately high zinc supply
Author: Tiong, J.
McDonald, G.
Genc, Y.
Pedas, P.
Hayes, J.
Toubia, J.
Langridge, P.
Huang, C.
Citation: New Phytologist, 2014; 201(1):131-143
Publisher: Wiley
Issue Date: 2014
ISSN: 0028-646X
Statement of
Jingwen Tiong, Glenn K. McDonald, Yusuf Genc, Pai Pedas, Julie E. Hayes, John Toubia, Peter Langridge, and Chun Y. Huang
Abstract: High expression of zinc (Zn)-regulated, iron-regulated transporter-like protein (ZIP) genes increases root Zn uptake in dicots, leading to high accumulation of Zn in shoots. However, none of the ZIP genes tested previously in monocots could enhance shoot Zn accumulation. In this report, barley (Hordeum vulgare) HvZIP7 was investigated for its functions in Zn transport. The functions of HvZIP7 in planta were studied using in situ hybridization and transient analysis of subcellular localization with a green fluorescent protein (GFP) reporter. Transgenic barley lines overexpressing HvZIP7 were also generated to further understand the functions of HvZIP7 in metal transport. HvZIP7 is strongly induced by Zn deficiency, primarily in vascular tissues of roots and leaves, and its protein was localized in the plasma membrane. These properties are similar to its closely related homologs in dicots. Overexpression of HvZIP7 in barley plants increased Zn uptake when moderately high concentrations of Zn were supplied. Significantly, there was a specific enhancement of shoot Zn accumulation, with no measurable increase in iron (Fe), manganese (Mn), copper (Cu) or cadmium (Cd). HvZIP7 displays characteristics of low-affinity Zn transport. The unique function of HvZIP7 provides new insights into the role of ZIP genes in Zn homeostasis in monocots, and offers opportunities to develop Zn biofortification strategies in cereals.
Keywords: accumulation; Hordeum vulgare (barley); low affinity; translocation; transporter; uptake; zinc (Zn); ZIP
Rights: © 2013 Australian Centre for Plant Functional Genomics
RMID: 0020133497
DOI: 10.1111/nph.12468
Appears in Collections:Agriculture, Food and Wine publications

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