Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/90318
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Type: Journal article
Title: kakapo, a gene required for adhesion between and within cell layers in Drosophila, encodes a large cytoskeletal linker protein related to plectin and dystrophin
Author: Gregory, S.
Brown, N.
Citation: The Journal of Cell Biology, 1998; 143(5):1271-1282
Publisher: Rockefeller University Press
Issue Date: 1998
ISSN: 0021-9525
1540-8140
Statement of
Responsibility: 
Stephen L. Gregory and Nicholas H. Brown
Abstract: Mutations in kakapo were recovered in genetic screens designed to isolate genes required for integrin-mediated adhesion in Drosophila. We cloned the gene and found that it encodes a large protein, amino acids, that is highly similar to plectin and BPAG, over the first, amino acid region, and contains within this region an α-actinin type actin-binding domain. A central region containing dystrophin-like repeats is followed by a carboxy domain that is distinct from plectin and dystrophin, having neither the intermediate filament-binding domain of plectin nor the dystroglycan, syntrophin-binding domain of dystrophin. Instead, Kakapo has a carboxy terminus similar to the growth arrest, specific protein Gas, . Kakapo is strongly expressed late during embryogenesis at the most prominent site of position-specific integrin adhesion, the muscle attachment sites. It is concentrated at apical and basal surfaces of epidermal muscle attachment cells, at the termini of the prominent microtubule bundles, and is required in these cells for strong attachment to muscles. Kakapo is also expressed more widely at a lower level where it is essential for epidermal cell layer stability. These results suggest that the Kakapo protein forms essential links among integrins, actin, and microtubules.
Keywords: integrins; cell adhesion; Drosophila; cytoskeleton; extracellular matrix
Rights: © The Rockefeller University Press
RMID: 0030013036
DOI: 10.1083/jcb.143.5.1271
Appears in Collections:Genetics publications

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