Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/91317
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DC Field | Value | Language |
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dc.contributor.author | Kant, K. | - |
dc.contributor.author | Yoo, J. | - |
dc.contributor.author | Amos, S. | - |
dc.contributor.author | Erkelens, M. | - |
dc.contributor.author | Priest, C. | - |
dc.contributor.author | Shapter, J. | - |
dc.contributor.author | Losic, D. | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | RSC Advances: an international journal to further the chemical sciences, 2015; 5(30):23886-23891 | - |
dc.identifier.issn | 2046-2069 | - |
dc.identifier.issn | 2046-2069 | - |
dc.identifier.uri | http://hdl.handle.net/2440/91317 | - |
dc.description.abstract | This paper presents a microfluidic device with a nano-channel prepared by focused ion beam (FIB) milling for microbial cell lysis and nucleic acid extraction. The device prepared in a quartz slide combines two microchannels connected with one nanochannel in the middle with dimensions of 100 nm (width) and 250 nm (depth) and 1 μm (length). The capturing of a single or a few cells and their lysis for extraction of DNA in nanochannel were demonstrated. The purity and quantity of the extracted genetic material was tested using a UV spectrometer. The results revealed that the extraction methodology was successful and may be well-suited to integration in analytical lab-on-a-chip devices. | - |
dc.description.statementofresponsibility | Krishna Kant, Jeongha Yoo, Steven Amos, Mason Erkelens, Craig Priest, Joe G. Shapter and Dusan Losic | - |
dc.language.iso | en | - |
dc.publisher | Royal Society of Chemistry | - |
dc.source.uri | http://dx.doi.org/10.1039/c5ra01336d | - |
dc.title | Microbial cell lysis and nucleic acid extraction via nanofluidic channel | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1039/c5ra01336d | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/FT110100711 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Losic, D. [0000-0002-1930-072X] | - |
Appears in Collections: | Aurora harvest 2 Chemical Engineering publications |
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