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Type: Journal article
Title: Effects of Escherichia coli Subtilase cytotoxin and Shiga toxin 2 on primary cultures of human renal tubular epithelial cells
Author: Marquez, L.
Velazquez, N.
Repetto, H.
Paton, A.
Paton, J.
Ibarra, C.
Silberstein, C.
Citation: PLoS One, 2014; 9(1):e87022-1-e87022-7
Publisher: Public Library of Science
Issue Date: 2014
ISSN: 1932-6203
Statement of
Laura B. Márquez, Natalia Velázquez, Horacio A. Repetto, Adrienne W. Paton, James C. Paton, Cristina Ibarra, Claudia Silberstein
Abstract: Shiga toxin (Stx)-producing Escherichia coli (STEC) cause post-diarrhea Hemolytic Uremic Syndrome (HUS), which is the most common cause of acute renal failure in children in many parts of the world. Several non-O157 STEC strains also produce Subtilase cytotoxin (SubAB) that may contribute to HUS pathogenesis. The aim of the present work was to examine the cytotoxic effects of SubAB on primary cultures of human cortical renal tubular epithelial cells (HRTEC) and compare its effects with those produced by Shiga toxin type 2 (Stx2), in order to evaluate their contribution to renal injury in HUS. For this purpose, cell viability, proliferation rate, and apoptosis were assayed on HRTEC incubated with SubAB and/or Stx2 toxins. SubAB significantly reduced cell viability and cell proliferation rate, as well as stimulating cell apoptosis in HRTEC cultures in a time dependent manner. However, HRTEC cultures were significantly more sensitive to the cytotoxic effects of Stx2 than those produced by SubAB. No synergism was observed when HRTEC were co-incubated with both SubAB and Stx2. When HRTEC were incubated with the inactive SubAA272B toxin, results were similar to those in untreated control cells. Similar stimulation of apoptosis was observed in Vero cells incubated with SubAB or/and Stx2, compared to HRTEC. In conclusion, primary cultures of HRTEC are significantly sensitive to the cytotoxic effects of SubAB, although, in a lesser extent compared to Stx2.
Keywords: Kidney Tubules
Description: Published: January 21, 2014
Rights: © 2014 Márquez et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0030008802
DOI: 10.1371/journal.pone.0087022
Appears in Collections:Molecular and Biomedical Science publications

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