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Type: Journal article
Title: Distribution, structure and biosynthetic gene families of (1,3;1,4)-β-glucan in Sorghum bicolor
Other Titles: Distribution, structure and biosynthetic gene families of (1,3;1,4)-beta-glucan in Sorghum bicolor
Author: Ermawar, R.
Collins, H.
Byrt, C.
Betts, N.
Henderson, M.
Shirley, N.
Schwerdt, J.
Lahnstein, J.
Fincher, G.
Burton, R.
Citation: Journal of Integrative Plant Biology, 2015; 57(4):429-445
Publisher: Wiley
Issue Date: 2015
ISSN: 1672-9072
Statement of
Riksfardini A. Ermawar, Helen M. Collins, Caitlin S. Byrt, Natalie S. Betts, Marilyn Henderson, Neil J. Shirley, Julian Schwerdt, Jelle Lahnstein, Geoffrey B. Fincher and Rachel A. Burton
Abstract: In cereals, the presence of soluble polysaccharides including (1,3;1,4)-β-glucan has downstream implications for human health, animal feed and biofuel applications. Sorghum bicolor (L.) Moench is a versatile crop, but there are limited reports regarding the content of such soluble polysaccharides. Here, the amount of (1,3;1,4)-β-glucan present in sorghum tissues was measured using a Megazyme assay. Very low amounts were present in the grain, ranging from 0.16%-0.27% (w/w), while there was a greater quantity in vegetative tissues at 0.12-1.71% (w/w). The fine structure of (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues, was assessed by high performance liquid chromatography (HPLC) and ranged from 2.6-3:1 in the grain, while ratios in vegetative tissues were lower at 2.1-2.6:1. The distribution of (1,3;1,4)-β-glucan was examined using a specific antibody and observed with fluorescence and transmission electron microscopy. Micrographs showed a variable distribution of (1,3;1,4)-β-glucan influenced by temporal and spatial factors. The sorghum orthologs of genes implicated in the synthesis of (1,3;1,4)-β-glucan in other cereals, such as the Cellulose synthase-like (Csl) F and H gene families were defined. Transcript profiling of these genes across sorghum tissues was carried out using real-time quantitative polymerase chain reaction, indicating that, as in other cereals, CslF6 transcripts dominated.
Keywords: Cellulose synthase-like genes; cell wall composition; grain; mixed-linkage glucan; sorghum
Description: Article first published online: 31 MAR 2015
Rights: © 2015 Institute of Botany, Chinese Academy of Sciences
RMID: 0030022648
DOI: 10.1111/jipb.12338
Appears in Collections:Agriculture, Food and Wine publications

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