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https://hdl.handle.net/2440/93029
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Type: | Journal article |
Title: | Total synthesis of homogeneous variants of hirudin P6: a post-translationally modified anti-thrombotic leech-derived protein |
Author: | Hsieh, Y. Wijeyewickrema, L. Wilkinson, B. Pike, R. Payne, R. |
Citation: | Angewandte Chemie International Edition, 2014; 53(15):3947-3951 |
Publisher: | Wiley-VCH Verlag |
Issue Date: | 2014 |
ISSN: | 1433-7851 1521-3773 |
Statement of Responsibility: | Yves S. Y. Hsieh, Lakshmi C. Wijeyewickrema, Brendan L. Wilkinson, Robert N. Pike, and Richard J. Payne |
Abstract: | Hirudin P6 is a leech-derived anti-thrombotic protein which possesses two post-translational modifications, O-glycosylation and tyrosine sulfation. In this study we report the ligation-based synthesis of a library of hirudin P6 proteins possessing homogeneous glycosylation and sulfation modifications. The nature of the modifications incorporated was shown to have a drastic effect on inhibition against both the fibrinogenolytic and amidolytic activities of thrombin and thus highlights a potential means for attenuating the biological activity of the protein. |
Keywords: | glycoprotein; glycosylation; ligation; sulfation; sulfoprotein |
Description: | Article first published online: 11 MAR 2014 |
Rights: | © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
DOI: | 10.1002/anie.201310777 |
Grant ID: | http://purl.org/au-research/grants/arc/FT130100150 |
Published version: | http://dx.doi.org/10.1002/anie.201310777 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 7 |
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