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Type: Journal article
Title: Salicylic acid improves salinity tolerance in Arabidopsis by restoring membrane potential and preventing salt-induced K⁺ loss via a GORK channel
Other Titles: Salicylic acid improves salinity tolerance in Arabidopsis by restoring membrane potential and preventing salt-induced K(+) loss via a GORK channel
Author: Jayakannan, M.
Bose, J.
Babourina, O.
Rengel, Z.
Shabala, S.
Citation: Journal of Experimental Botany, 2013; 64(8):2255-2268
Publisher: Oxford University Press (OUP)
Issue Date: 2013
ISSN: 0022-0957
Statement of
Maheswari Jayakannan, Jayakumar Bose, Olga Babourina, Zed Rengel, and Sergey Shabala
Abstract: Despite numerous reports implicating salicylic acid (SA) in plant salinity responses, the specific ionic mechanisms of SA-mediated adaptation to salt stress remain elusive. To address this issue, a non-invasive microelectrode ion flux estimation technique was used to study kinetics of NaCl-induced net ion fluxes in Arabidopsis thaliana in response to various SA concentrations and incubation times. NaCl-induced K+ efflux and H+ influx from the mature root zone were both significantly decreased in roots pretreated with 10–500 μM SA, with strongest effect being observed in the 10–50 μM SA range. Considering temporal dynamics (0–8-h SA pretreatment), the 1-h pretreatment was most effective in enhancing K+ retention in the cytosol. The pharmacological, membrane potential, and shoot K+ and Na+ accumulation data were all consistent with the model in which the SA pretreatment enhanced activity of H+-ATPase, decreased NaCl-induced membrane depolarization, and minimized NaCl-induced K+ leakage from the cell within the first hour of salt stress. In long-term treatments, SA increased shoot K+ and decreased shoot Na+ accumulation. The short-term NaCl-induced K+ efflux was smallest in the gork1-1 mutant, followed by the rbohD mutant, and was highest in the wild type. Most significantly, the SA pretreatment decreased the NaCl-induced K+ efflux from rbohD and the wild type to the level of gork1-1, whereas no effect was observed in gork1-1. These data provide the first direct evidence that the SA pretreatment ameliorates salinity stress by counteracting NaCl-induced membrane depolarization and by decreasing K+ efflux via GORK channels.
Keywords: Arabidopsis; Plant Shoots; Plant Roots; Sodium Chloride; Potassium; Salicylic Acid; Plant Growth Regulators; Potassium Channels; Membrane Potentials; Dose-Response Relationship, Drug; Salt-Tolerance
Rights: © The Author(2) [2013]. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0030031568
DOI: 10.1093/jxb/ert085
Appears in Collections:Agriculture, Food and Wine publications

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