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Type: Journal article
Title: The biochemical mechanism of caspase-2 activation
Author: Baliga, B.
Read, S.
Kumar, S.
Citation: Cell Death and Differentiation, 2004; 11(11):1234-1241
Publisher: Nature Publishing Group
Issue Date: 2004
ISSN: 1350-9047
Statement of
B C Baliga, S H Read and S Kumar
Abstract: A unified model for initiator caspase activation has previously been proposed based on the biochemical analysis of caspase-8 and -9. Caspase-2 is structurally related to caspase-9, but its mechanism of activation is not known. Using an uncleavable mutant of caspase-2, we show that dimerization (and not processing) is the key event that drives initial procaspase-2 activation. Following dimerization, caspase-2 undergoes autocatalytic cleavage that promotes its stable dimerization and further enhances the catalytic activity of caspase-2. Although the caspase-2 zymogen does not require cleavage for the initial acquisition of activity, intersubunit cleavage is required to generate levels of activity required to induce cell death by overexpression. We also provide evidence that the reported disulfide bond linkage between two caspase-2 monomers is dispensable for caspase-2 dimerization. As caspase-2 does not require cleavage for its initial activation, our findings confirm caspase-2 to be a bona fide initiator caspase.
Keywords: Cell Line; Animals; Humans; Disulfides; Caspases; Glutathione Transferase; Recombinant Fusion Proteins; Chromatography; Electrophoresis, Polyacrylamide Gel; Polymerase Chain Reaction; Apoptosis; Protein Biosynthesis; Enzyme Activation; Protein Structure, Tertiary; Protein Binding; Dimerization; Mutation; Catalysis; Caspase 2
Description: Copyright © 2004 Nature Publishing Group
Provenance: Published online 6 August 2004
RMID: 0020040694
DOI: 10.1038/sj.cdd.4401492
Appears in Collections:Medicine publications

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