Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/95588
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Factors derived from escherichia coli nissle 1917, grown in different growth media, enhance cell death in a model of 5-fluorouracil-induced caco-2 intestinal epithelial cell damage
Author: Wang, H.
Bastian, S.
Lawrence, A.
Howarth, G.
Citation: Nutrition and Cancer, 2015; 67(2):316-326
Publisher: Taylor & Francis
Issue Date: 2015
ISSN: 0163-5581
1532-7914
Statement of
Responsibility: 
Hanru Wang, Susan E. P. Bastian, Andrew Lawrence & Gordon S. Howarth
Abstract: We evaluated supernatants (SNs) from Escherichia coli Nissle 1917 (EcN) grown in commonly used growth media for their capacity to affect the viability of Caco-2 colon cancer cells in the presence and absence of 5-Fluorouracil (5-FU) chemotherapy. EcN was grown in Luria-Bertani (LB), tryptone soya (TSB), Man Rogosa Sharpe (MRS), and M17 broth supplemented with 10% (v/v) lactose solution (M17). Human Caco-2 colon cancer cells were treated with DMEM (control), growth media alone (LB, TSB, MRS, and M17) or EcN SNs derived from these 4 media, in the presence and absence of 5-FU. Cell viability, reactive oxygen species (ROS), and cell monolayer permeability were determined. EcN SN in LB medium reduced Caco-2 cell viability significantly, to 51% at 48 h. The combination of this EcN SN and 5-FU further reduced cell viability to 37% at 48 h, compared to 5-FU control. MRS broth and EcN SN in MRS, together with 5-FU, generated significantly lower levels of ROS compared to 5-FU control. However, all 5-FU treatments significantly disrupted the Caco-2 cell barrier compared to control; with no significant differences observed among any of the 5-FU treatments. EcN SNs (LB+) was most effective at decreasing the viability of Caco-2 cells. This could indicate a potential role for this EcN SN in chemoprevention for colon cancer.
Keywords: Caco-2 Cells; Humans; Escherichia coli; Colonic Neoplasms; Reactive Oxygen Species; Fluorouracil; Escherichia coli Proteins; Culture Media; Cell Death; Cell Membrane Permeability; Cell Survival; Electric Impedance
Rights: © 2015, Taylor & Francis Group, LLC
RMID: 0030021729
DOI: 10.1080/01635581.2015.990570
Appears in Collections:Agriculture, Food and Wine publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.