Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Quantifying two-dimensional filamentous and invasive growth spatial patterns in yeast colonies
Author: Binder, B.
Sundstrom, J.
Gardner, J.
Jiranek, V.
Oliver, S.
Citation: PLoS Computational Biology, 2015; 11(2):e1004070-1-e1004070-15
Publisher: Public Library of Science
Issue Date: 2015
ISSN: 1553-7358
Editor: Lauffenburger, D.
Statement of
Benjamin J. Binder, Joanna F. Sundstrom, Jennifer M. Gardner, Vladimir Jiranek, Stephen G. Oliver
Abstract: The top-view, two-dimensional spatial patterning of non-uniform growth in a Saccharomyces cerevisiae yeast colony is considered. Experimental images are processed to obtain data sets that provide spatial information on the cell-area that is occupied by the colony. A method is developed that allows for the analysis of the spatial distribution with three metrics. The growth of the colony is quantified in both the radial direction from the centre of the colony and in the angular direction in a prescribed outer region of the colony. It is shown that during the period of 100-200 hours from the start of the growth of the colony there is an increasing amount of non-uniform growth. The statistical framework outlined in this work provides a platform for comparative quantitative assays of strain-specific mechanisms, with potential implementation in inferencing algorithms used for parameter-rate estimation.
Keywords: Saccharomyces cerevisiae
Computational Biology
Models, Biological
Image Processing, Computer-Assisted
Rights: © 2015 Binder et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
DOI: 10.1371/journal.pcbi.1004070
Grant ID:
Appears in Collections:Aurora harvest 3
Mathematical Sciences publications

Files in This Item:
File Description SizeFormat 
hdl_97188.pdfPublished version3.19 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.