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https://hdl.handle.net/2440/97970
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Type: | Journal article |
Title: | Molecular interaction of the γ-clade homeodomain-leucine zipper class I transcription factors during the wheat response to water deficit |
Other Titles: | Molecular interaction of the gamma-clade homeodomain-leucine zipper class I transcription factors during the wheat response to water deficit |
Author: | Harris, J. Sornaraj, P. Taylor, M. Bazanova, N. Baumann, U. Lovell, B. Langridge, P. Lopato, S. Hrmova, M. |
Citation: | Plant Molecular Biology: An International Journal on Molecular Biology, Molecular Genetics and Biochemistry, 2016; 90(4):435-452 |
Publisher: | Springer |
Issue Date: | 2016 |
ISSN: | 0167-4412 1573-5028 |
Statement of Responsibility: | John C. Harris, Pradeep Sornaraj, Mathew Taylor, Natalia Bazanova, Ute Baumann, Ben Lovell, Peter Langridge, Sergiy Lopato, Maria Hrmova |
Abstract: | The ᵧ-clade of class I homeodomain-leucine zipper (HD-Zip I) transcription factors (TFs) constitute members which play a role in adapting plant growth to conditions of water deficit. Given the importance of wheat (Triticum aestivum L.) as a global food crop and the impact of water deficit upon grain yield, we focused on functional aspects of wheat drought responsive HD-Zip I TFs. While the wheat ᵧ-clade HD-Zip I TFs share significant sequence similarities with homologous genes from other plants, the clade-specific features in transcriptional response to abiotic stress were detected. We demonstrate that wheat TaHDZipI- 3, TaHDZipI-4, and TaHDZipI-5 genes respond differentially to a variety of abiotic stresses, and that proteins encoded by these genes exhibit pronounced differences in oligomerisation, strength of DNA binding, and trans-activation of an artificial promoter. Three-dimensional molecular modelling of the protein-DNA interface was conducted to address the ambiguity at the central nucleotide in the pseudo-palindromic cis-element CAATNATTG that is recognised by all three HD-Zip I proteins. The coexpression of these genes in the same plant tissues together with the ability of HD-Zip I TFs of the ᵧ -clade to heterodimerise suggests a role in the regulatory mechanisms of HD-Zip I dependent transcription. Our findings highlight the complexity of TF networks involved in plant responses to water deficit. A better understanding of the molecular complexity at the protein level during crop responses to drought will enable adoption of efficient strategies for production of cereal plants with enhanced drought tolerance. |
Keywords: | Abiotic stres; Homeodomain leucine zipper; Transcription factor networks; Homo- and heterodimerisation; DNA binding; Molecular modelling |
Rights: | © Springer Science+Business Media Dordrecht 2016 |
DOI: | 10.1007/s11103-015-0427-6 |
Grant ID: | ARC |
Published version: | http://dx.doi.org/10.1007/s11103-015-0427-6 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 3 |
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File | Description | Size | Format | |
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hdl_97970.pdf | Accepted version | 391.96 kB | Adobe PDF | View/Open |
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