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Type: Journal article
Title: Molecular interaction of the γ-clade homeodomain-leucine zipper class I transcription factors during the wheat response to water deficit
Other Titles: Molecular interaction of the gamma-clade homeodomain-leucine zipper class I transcription factors during the wheat response to water deficit
Author: Harris, J.
Sornaraj, P.
Taylor, M.
Bazanova, N.
Baumann, U.
Lovell, B.
Langridge, P.
Lopato, S.
Hrmova, M.
Citation: Plant Molecular Biology, 2016; 90(4):435-452
Publisher: Springer
Issue Date: 2016
ISSN: 0167-4412
Statement of
John C. Harris, Pradeep Sornaraj, Mathew Taylor, Natalia Bazanova, Ute Baumann, Ben Lovell, Peter Langridge, Sergiy Lopato, Maria Hrmova
Abstract: The ᵧ-clade of class I homeodomain-leucine zipper (HD-Zip I) transcription factors (TFs) constitute members which play a role in adapting plant growth to conditions of water deficit. Given the importance of wheat (Triticum aestivum L.) as a global food crop and the impact of water deficit upon grain yield, we focused on functional aspects of wheat drought responsive HD-Zip I TFs. While the wheat ᵧ-clade HD-Zip I TFs share significant sequence similarities with homologous genes from other plants, the clade-specific features in transcriptional response to abiotic stress were detected. We demonstrate that wheat TaHDZipI- 3, TaHDZipI-4, and TaHDZipI-5 genes respond differentially to a variety of abiotic stresses, and that proteins encoded by these genes exhibit pronounced differences in oligomerisation, strength of DNA binding, and trans-activation of an artificial promoter. Three-dimensional molecular modelling of the protein-DNA interface was conducted to address the ambiguity at the central nucleotide in the pseudo-palindromic cis-element CAATNATTG that is recognised by all three HD-Zip I proteins. The coexpression of these genes in the same plant tissues together with the ability of HD-Zip I TFs of the ᵧ -clade to heterodimerise suggests a role in the regulatory mechanisms of HD-Zip I dependent transcription. Our findings highlight the complexity of TF networks involved in plant responses to water deficit. A better understanding of the molecular complexity at the protein level during crop responses to drought will enable adoption of efficient strategies for production of cereal plants with enhanced drought tolerance.
Keywords: Abiotic stres; Homeodomain leucine zipper; Transcription factor networks; Homo- and heterodimerisation; DNA binding; Molecular modelling
Rights: © Springer Science+Business Media Dordrecht 2016
RMID: 0030041935
DOI: 10.1007/s11103-015-0427-6
Appears in Collections:Agriculture, Food and Wine publications

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