Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/98058
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Type: Journal article
Title: Isolation site influences virulence phenotype of serotype 14 Streptococcus pneumoniae strains belonging to multilocus sequence type 15
Author: Amin, Z.
Harvey, R.
Wang, H.
Hughes, C.
Paton, A.
Paton, J.
Trappetti, C.
Citation: Infection and Immunity, 2015; 83(12):4781-4790
Publisher: American Society for Microbiology
Issue Date: 2015
ISSN: 0019-9567
1098-5522
Statement of
Responsibility: 
Zarina Amin, Richard M. Harvey, Hui Wang, Catherine E. Hughes, Adrienne W. Paton, James C. Paton, Claudia Trappetti
Abstract: Streptococcus pneumoniae is a diverse species causing invasive as well as localized infections that result in massive global morbidity and mortality. Strains vary markedly in pathogenic potential, but the molecular basis is obscured by the diversity and plasticity of the pneumococcal genome. We have previously reported that S. pneumoniae serotype 3 isolates belonging to the same multilocus sequence type (MLST) differed markedly in in vitro and in vivo phenotypes, in accordance with the clinical site of isolation, suggesting stable niche adaptation within a clonal lineage. In the present study, we have extended our analysis to serotype 14 clinical isolates from cases of sepsis or otitis media that belong to the same MLST (ST15). In a murine intranasal challenge model, five ST15 isolates (three from blood and two from ears) colonized the nasopharynx to similar extents. However, blood and ear isolates exhibited significant differences in bacterial loads in other host niches (lungs, ear, and brain) at both 24 and 72 h postchallenge. In spite of these differences, blood and ear isolates were present in the lungs at similar levels at 6 h postchallenge, suggesting that early immune responses may underpin the distinct virulence phenotypes. Transcriptional analysis of lung tissue from mice infected for 6 h with blood isolates versus ear isolates revealed 8 differentially expressed genes. Two of these were exclusively expressed in response to infection with the ear isolate. These results suggest a link between the differential capacities to elicit early innate immune responses and the distinct virulence phenotypes of clonally related S. pneumoniae strains.
Keywords: Ear; Lung; Nasopharynx; Clone Cells; Animals; Mice; Streptococcus pneumoniae; Pneumococcal Infections; Sepsis; Otitis Media; Serotyping; Gene Expression Profiling; Virulence; Signal Transduction; Gene Expression Regulation; Phenotype; Genome, Bacterial; Female; Host-Pathogen Interactions; Immunity, Innate; Multilocus Sequence Typing
Rights: Copyright © 2015, American Society for Microbiology. All Rights Reserved
RMID: 0030037137
DOI: 10.1128/IAI.01081-15
Appears in Collections:Molecular and Biomedical Science publications

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