Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/98125
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Type: Journal article
Title: Influence of aromatase absence on the gene expression and histology of the mouse meibomian gland
Author: Rahimi Darabad, R.
Suzuki, T.
Richards, S.
Jensen, R.
Jakobiec, F.
Zakka, F.
Liu, S.
Sullivan, D.
Citation: Investigative Opthalmology & Visual Science, 2013; 54(2):987-998
Publisher: Mosby
Issue Date: 2013
ISSN: 0146-0404
1552-5783
Statement of
Responsibility: 
Raheleh Rahimi Darabad, Tomo Suzuki, Stephen M. Richards, Roderick V. Jensen, Frederick A. Jakobiec, Fouad R. Zakka, Shaohui Liu and David A. Sullivan
Abstract: Purpose.: We hypothesize that aromatase, an enzyme that controls estrogen biosynthesis, plays a major role in the sex-related differences of the meibomian gland. To begin to test this hypothesis, we examined the influence of aromatase absence, which completely eliminates estrogen production, on glandular gene expression and histology in male and female mice. Methods.: Meibomian glands were obtained from adult, age-matched wild-type (WT) and aromatase knockout (ArKO) mice. Tissues were processed for histology or the isolation of total RNA, which was analyzed for differentially expressed mRNAs by using microarrays. Results.: Our results show that aromatase significantly influences the expression of more than a thousand genes in the meibomian gland. The nature of this effect is primarily sex-dependent. In addition, the influence of aromatase on sex-related differences in gene expression is predominantly genotype-specific. However, many of the sex-related variations in biological process, molecular function, and cellular component ontologies, as well as in KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways, are remarkably similar between WT and ArKO mice. The loss of aromatase activity has no obvious effect on the histology of meibomian glands in male or female mice. Conclusions.: Our findings demonstrate that aromatase has a significant impact on gene expression in the meibomian gland. The nature of this influence is sex-dependent and genotype-specific; however, many of the sex-related variations in gene ontologies and KEGG pathways are similar between WT and ArKO mice. Consequently, it appears that aromatase, and by extension estrogen, do not play a major role in the sex-related differences of the mouse meibomian gland.
Rights: Copyright 2013 The Association for Research in Vision and Ophthalmology, Inc.
RMID: 0030042576
DOI: 10.1167/iovs.12-10992
Appears in Collections:Genetics publications

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