Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/98801
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Type: Journal article
Title: Major improvements to the Heliconius melpomene genome assembly used to confirm 10 chromosome fusion events in 6 million years of butterfly evolution
Author: Davey, J.
Chouteau, M.
Barker, S.
Maroja, L.
Baxter, S.
Simpson, F.
Merrill, R.
Joron, M.
Mallet, J.
Dasmahapatra, K.
Jiggins, C.
Citation: G3: Genes, Genomes, Genetics, 2016; 6(3):695-708
Publisher: Genetics Society of America
Issue Date: 2016
ISSN: 2160-1836
2160-1836
Statement of
Responsibility: 
John W. Davey, Mathieu Chouteau, Sarah L. Barker, Luana Maroja, Simon W. Baxter, Fraser Simpson, Richard M. Merrill, Mathieu Joron, James Mallet, Kanchon K. Dasmahapatra and Chris D. Jiggins
Abstract: The Heliconius butterflies are a widely studied adaptive radiation of 46 species spread across Central and South America, several of which are known to hybridize in the wild. Here, we present a substantially improved assembly of the Heliconius melpomene genome, developed using novel methods that should be applicable to improving other genome assemblies produced using short read sequencing. First, we whole-genome-sequenced a pedigree to produce a linkage map incorporating 99% of the genome. Second, we incorporated haplotype scaffolds extensively to produce a more complete haploid version of the draft genome. Third, we incorporated ∼20x coverage of Pacific Biosciences sequencing, and scaffolded the haploid genome using an assembly of this long-read sequence. These improvements result in a genome of 795 scaffolds, 275 Mb in length, with an N50 length of 2.1 Mb, an N50 number of 34, and with 99% of the genome placed, and 84% anchored on chromosomes. We use the new genome assembly to confirm that the Heliconius genome underwent 10 chromosome fusions since the split with its sister genus Eueides, over a period of about 6 million yr.
Keywords: Heliconius; genome assembly; linkage mapping; chromosome fusions; Eueides
Rights: © 2016 Davey et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0030042321
DOI: 10.1534/g3.115.023655
Appears in Collections:Genetics publications

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