Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/99187
Citations
Scopus Web of Science® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorStewart, J.-
dc.contributor.authorCowan, J.-
dc.contributor.authorPerry, L.-
dc.contributor.authorColdwell, M.-
dc.contributor.authorProud, C.-
dc.date.issued2015-
dc.identifier.citationBiochemical Journal, 2015; 467(2):217-229-
dc.identifier.issn0264-6021-
dc.identifier.issn1470-8728-
dc.identifier.urihttp://hdl.handle.net/2440/99187-
dc.description.abstractATP-binding cassette 50 (ABC50; also known as ABCF1) binds to eukaryotic initiation factor 2 (eIF2) and is required for efficient translation initiation. An essential step of this process is accurate recognition and selection of the initiation codon. It is widely accepted that the presence and movement of eIF1, eIF1A and eIF5 are key factors in modulating the stringency of start-site selection, which normally requires an AUG codon in an appropriate sequence context. In the present study, we show that expression of ABC50 mutants, which cannot hydrolyse ATP, decreases general translation and relaxes the discrimination against the use of non-AUG codons at translation start sites. These mutants do not appear to alter the association of key initiation factors to 40S subunits. The stringency of start-site selection can be restored through overexpression of eIF1, consistent with the role of that factor in enhancing stringency. The present study indicates that interfering with the function of ABC50 influences the accuracy of initiation codon selection.-
dc.description.statementofresponsibilityJoanna D. Stewart, Joanne L. Cowan, Lisa S. Perry, Mark J. Coldwell and Christopher G. Proud-
dc.language.isoen-
dc.publisherPortland Press-
dc.rights© The Authors. Journal compilation © 2015 Biochemical Society-
dc.source.urihttp://dx.doi.org/10.1042/bj20141453-
dc.subjectTranslation initiation; eIF2; ABCF1; Start codon; Accuracy; Initiation factor-
dc.titleABC50 mutants modify translation start codon selection-
dc.typeJournal article-
dc.identifier.doi10.1042/BJ20141453-
pubs.publication-statusPublished-
dc.identifier.orcidProud, C. [0000-0003-0704-6442]-
Appears in Collections:Aurora harvest 7
Biochemistry publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.