Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/9921
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Type: Journal article
Title: Determination of L-arginine and NG,NG- and NG,NG'-dimethyl-L-arginine in plasma by liquid chromatography as AccQ-FluorTM fluorescent derivatives
Author: Heresztyn, T.
Worthley, M.
Horowitz, J.
Citation: Journal of Chromatography. B, Analytical Techniques in the Biomedical and Life Sciences, 2004; 805(2):325-329
Publisher: Elsevier Science BV
Issue Date: 2004
ISSN: 1570-0232
1873-376X
Statement of
Responsibility: 
Tamila Heresztyn, Matthew I. Worthley, John D. Horowitz
Abstract: A new HPLC assay for the detection of L-arginine, NG, NG-dimethyl-L-arginine (ADMA) and NG, NG' -dimethyl-L-arginine (SDMA) in plasma using the derivatisation reagent AccQ-Fluor (6-aminoquinolyl-N-hydroxysuccinimidyl carbamate) is described. The fluorescent derivatives produced are extremely stable enabling routine processing of large numbers of samples. Arginine and its metabolites are extracted from plasma on strong cation exchange (SCX) cartridges with NG-monomethyl-L-arginine (NMMA) as internal standard, derivatised and separated on a C18 column with acetonitrile in 0.1M sodium acetate buffer pH 6. Separation of the stereoisomers ADMA and SDMA was excellent and improvements to the solid phase extraction (SPE) procedure enabled good recovery (>80%) of arginine, ADMA and SDMA. The utility of the method is exemplified by comparison of plasma concentrations of ADMA, SDMA and arginine in healthy volunteers and diabetic/ischaemic patients.
Keywords: Derivatisation, LC; L-Arginine; Dimethyl-L-arginine
Description: © 2004 Elsevier B.V. All rights reserved.
RMID: 0020040467
DOI: 10.1016/j.jchromb.2004.03.020
Appears in Collections:Medicine publications

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