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Type: Journal article
Title: A barley efflux transporter operates in a Na⁺-dependent manner, as revealed by a multidisciplinary platform
Other Titles: A barley efflux transporter operates in a Na(+)-dependent manner, as revealed by a multidisciplinary platform
Author: Nagarajan, Y.
Rongala, J.
Luang, S.
Singh, A.
Shadiac, N.
Hayes, J.
Sutton, T.
Gilliham, M.
Tyerman, S.
McPhee, G.
Voelcker, N.
Mertens, H.
Kirby, N.
Lee, J.
Yingling, Y.
Hrmova, M.
Citation: The Plant Cell, 2016; 28(1):202-218
Publisher: American Society of Plant Biologists
Issue Date: 2016
ISSN: 1040-4651
Statement of
Yagnesh Nagarajan, Jay Rongala, Sukanya Luang, Abhishek Singh, Nadim Shadiac, Julie Hayes, Tim Sutton, Matthew Gilliham, Stephen D. Tyerman, Gordon McPhee, Nicolas H. Voelcker, Haydyn D.T. Mertens, Nigel M. Kirby, Jung-Goo Lee, Yaroslava G. Yingling, and Maria Hrmova
Abstract: Plant growth and survival depend upon the activity of membrane transporters that control the movement and distribution of solutes into, around, and out of plants. Although many plant transporters are known, their intrinsic properties make them difficult to study. In barley (Hordeum vulgare), the root anion-permeable transporter Bot1 plays a key role in tolerance to high soil boron, facilitating the efflux of borate from cells. However, its three-dimensional structure is unavailable and the molecular basis of its permeation function is unknown. Using an integrative platform of computational, biophysical, and biochemical tools as well as molecular biology, electrophysiology, and bioinformatics, we provide insight into the origin of transport function of Bot1. An atomistic model, supported by atomic force microscopy measurements, reveals that the protein folds into 13 transmembrane-spanning and five cytoplasmic α-helices. We predict a trimeric assembly of Bot1 and the presence of a Na(+) ion binding site, located in the proximity of a pore that conducts anions. Patch-clamp electrophysiology of Bot1 detects Na(+)-dependent polyvalent anion transport in a Nernstian manner with channel-like characteristics. Using alanine scanning, molecular dynamics simulations, and transport measurements, we show that conductance by Bot1 is abolished by removal of the Na(+) ion binding site. Our data enhance the understanding of the permeation functions of Bot1.
Keywords: Cell-Free System; Pichia; Hordeum; Triticum; Borates; Anions; Sodium; Lipid Bilayers; Membrane Transport Proteins; Plant Proteins; Liposomes; Binding Sites; Protein Folding; Permeability; Models, Molecular; Computer Simulation; Protein Multimerization
Rights: © 2016 American Society of Plant Biologists. All rights reserved.
RMID: 0030040529
DOI: 10.1105/tpc.15.00625
Grant ID:
Appears in Collections:Agriculture, Food and Wine publications

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