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|Title:||Cell-free synthesis of a functional membrane transporter into a tethered bilayer lipid membrane|
|Citation:||Langmuir, 2016; 32(10):2445-2449|
|Publisher:||American Chemical Society|
|Julius L. Zieleniecki, Yagnesh Nagarajan, Shane Waters, Jay Rongala, Vanessa Thompson, Maria Hrmova, and Ingo Köper|
|Abstract:||Eukaryotic cell-free synthesis was used to incorporate the large and complex multispan plant membrane transporter Bot1 in a functional form into a tethered bilayer lipid membrane. The electrical properties of the protein-functionalized tethered bilayer were measured using electrochemical impedance spectroscopy and revealed a pH-dependent transport of borate ions through the protein. The efficacy of the protein synthesis has been evaluated using immunoblot analysis.|
|Keywords:||Cell-Free System; Hordeum; Boric Acids; Esters; Thioctic Acid; Lipid Bilayers; Phosphatidylcholines; Membrane Transport Proteins; Plant Proteins; RNA, Messenger; RNA, Plant; Immunoblotting; Hydrogen-Ion Concentration; Dielectric Spectroscopy|
|Rights:||© 2016 American Chemical Society|
|Appears in Collections:||Agriculture, Food and Wine publications|
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