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Type: Journal article
Title: Cell-free synthesis of a functional membrane transporter into a tethered bilayer lipid membrane
Author: Zieleniecki, J.
Nagarajan, Y.
Waters, S.
Rongala, J.
Thompson, V.
Hrmova, M.
Köper, I.
Citation: Langmuir, 2016; 32(10):2445-2449
Publisher: American Chemical Society
Issue Date: 2016
ISSN: 0743-7463
Statement of
Julius L. Zieleniecki, Yagnesh Nagarajan, Shane Waters, Jay Rongala, Vanessa Thompson, Maria Hrmova, and Ingo Köper
Abstract: Eukaryotic cell-free synthesis was used to incorporate the large and complex multispan plant membrane transporter Bot1 in a functional form into a tethered bilayer lipid membrane. The electrical properties of the protein-functionalized tethered bilayer were measured using electrochemical impedance spectroscopy and revealed a pH-dependent transport of borate ions through the protein. The efficacy of the protein synthesis has been evaluated using immunoblot analysis.
Keywords: Cell-Free System; Hordeum; Boric Acids; Esters; Thioctic Acid; Lipid Bilayers; Phosphatidylcholines; Membrane Transport Proteins; Plant Proteins; RNA, Messenger; RNA, Plant; Immunoblotting; Hydrogen-Ion Concentration; Dielectric Spectroscopy
Rights: © 2016 American Chemical Society
RMID: 0030044070
DOI: 10.1021/acs.langmuir.5b04059
Grant ID:
Appears in Collections:Agriculture, Food and Wine publications

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