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Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/99860
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dc.contributor.authorZieleniecki, J.-
dc.contributor.authorNagarajan, Y.-
dc.contributor.authorWaters, S.-
dc.contributor.authorRongala, J.-
dc.contributor.authorThompson, V.-
dc.contributor.authorHrmova, M.-
dc.contributor.authorKöper, I.-
dc.date.issued2016-
dc.identifier.citationLangmuir: the ACS journal of surfaces and colloids, 2016; 32(10):2445-2449-
dc.identifier.issn0743-7463-
dc.identifier.issn1520-5827-
dc.identifier.urihttp://hdl.handle.net/2440/99860-
dc.description.abstractEukaryotic cell-free synthesis was used to incorporate the large and complex multispan plant membrane transporter Bot1 in a functional form into a tethered bilayer lipid membrane. The electrical properties of the protein-functionalized tethered bilayer were measured using electrochemical impedance spectroscopy and revealed a pH-dependent transport of borate ions through the protein. The efficacy of the protein synthesis has been evaluated using immunoblot analysis.-
dc.description.statementofresponsibilityJulius L. Zieleniecki, Yagnesh Nagarajan, Shane Waters, Jay Rongala, Vanessa Thompson, Maria Hrmova, and Ingo Köper-
dc.language.isoen-
dc.publisherAmerican Chemical Society-
dc.rights© 2016 American Chemical Society-
dc.source.urihttp://dx.doi.org/10.1021/acs.langmuir.5b04059-
dc.subjectCell-Free System-
dc.subjectHordeum-
dc.subjectBoric Acids-
dc.subjectEsters-
dc.subjectThioctic Acid-
dc.subjectLipid Bilayers-
dc.subjectPhosphatidylcholines-
dc.subjectMembrane Transport Proteins-
dc.subjectPlant Proteins-
dc.subjectRNA, Messenger-
dc.subjectRNA, Plant-
dc.subjectImmunoblotting-
dc.subjectHydrogen-Ion Concentration-
dc.subjectDielectric Spectroscopy-
dc.titleCell-free synthesis of a functional membrane transporter into a tethered bilayer lipid membrane-
dc.typeJournal article-
dc.identifier.doi10.1021/acs.langmuir.5b04059-
dc.relation.granthttp://purl.org/au-research/grants/arc/DP120100900-
pubs.publication-statusPublished-
dc.identifier.orcidHrmova, M. [0000-0002-3545-0605]-
Appears in Collections:Agriculture, Food and Wine publications
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