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https://hdl.handle.net/2440/100728
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Type: | Journal article |
Title: | MALDI mass spectrometry imaging reveals decreased CK5 levels in vulvar squamous cell carcinomas compared to the precursor lesion differentiated vulvar intraepithelial neoplasia |
Author: | Zhang, C. Arentz, G. Winderbaum, L. Lokman, N. Klingler-Hoffmann, M. Mittal, P. Carter, C. Oehler, M. Hoffmann, P. |
Citation: | International Journal of Molecular Sciences, 2016; 17(7):1088-1-1088-12 |
Publisher: | MDPI |
Issue Date: | 2016 |
ISSN: | 1661-6596 1422-0067 |
Statement of Responsibility: | Chao Zhang, Georgia Arentz, Lyron Winderbaum, Noor A. Lokman, Manuela Klingler-Hoffmann, Parul Mittal, Christopher Carter, Martin K. Oehler and Peter Hoffmann |
Abstract: | Vulvar cancer is the fourth most common gynecological cancer worldwide. However, limited studies have been completed on the molecular characterization of vulvar squamous cell carcinoma resulting in a poor understanding of the disease initiation and progression. Analysis and early detection of the precursor lesion of HPV-independent vulvar squamous cell carcinoma (VSCC), differentiated vulvar intraepithelial neoplasia (dVIN), is of great importance given dVIN lesions have a high level of malignant potential. Here we present an examination of adjacent normal vulvar epithelium, dVIN, and VSCC from six patients by peptide Matrix-assisted laser desorption/ionization Mass Spectrometry Imaging (MALDI-MSI). The results reveal the differential expression of multiple peptides from the protein cytokeratin 5 (CK5) across the three vulvar tissue types. The difference observed in the relative abundance of CK5 by MALDI-MSI between the healthy epithelium, dVIN, and VSCC was further analyzed by immunohistochemistry (IHC) in tissue from eight VSCC patients. A decrease in CK5 immunostaining was observed in the VSCC compared to the healthy epithelium and dVIN. These results provide an insight into the molecular fingerprint of the vulvar intraepithelial neoplasia that appears to be more closely related to the healthy epithelium than the VSCC. |
Keywords: | Matrix-assisted laser desorption/ionization (MALDI); imaging mass spectrometry (MSI); differentiated vulvar intraepithelial neoplasia (dVIN); vulva squamous cell carcinomas (VSCC); Cytokeratin 5 (CK5) |
Description: | Published: 8 July 2016 |
Rights: | © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
DOI: | 10.3390/ijms17071088 |
Published version: | http://dx.doi.org/10.3390/ijms17071088 |
Appears in Collections: | Aurora harvest 3 IPAS publications |
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hdl_100728.pdf | Published version | 1.42 MB | Adobe PDF | View/Open |
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