Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/103701
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Poly(N-isopropylacrylamide) hydrogel/chitosan scaffold hybrid for three-dimensional stem cell culture and cartilage tissue engineering |
Author: | Mellati, A. Kiamahalleh, M. Madani, S. Dai, S. Bi, J. Jin, B. Zhang, H. |
Citation: | Journal of Biomedical Materials Research Part A, 2016; 104(11):2764-2774 |
Publisher: | Wiley |
Issue Date: | 2016 |
ISSN: | 1552-4965 1552-4965 |
Statement of Responsibility: | Amir Mellati, Meisam Valizadeh Kiamahalleh, S. Hadi Madani, Sheng Dai, Jingxiu Bi, Bo Jin, Hu Zhang |
Abstract: | Providing a controllable and definable three-dimensional (3D) microenvironment for chondrogenic differentiation of mesenchymal stem cells (MSCs) remains a great challenge for cartilage tissue engineering. In this work, poly(N-isopropylacrylamide) (PNIPAAm) polymers with the degrees of polymerization of 100 and 400 (NI100 and NI400) were prepared and the polymer solutions were introduced into the preprepared chitosan porous scaffolds (CS) to form hybrids (CSNI100 and CSNI400, respectively). SEM images indicated that the PNIPAAm gel partially occupied chitosan pores while the interconnected porous structure of chitosan was preserved. MSCs were incorporated within the hybrid and cell proliferation and chondrogenic differentiation were monitored. After 7-day incubation of the cell-laden constructs in a growth medium, the cell viability in CSNI100 and CSNI400 were 54 and 108% higher than that in CS alone, respectively. Glycosaminoglycan and total collagen contents increased 2.6- and 2.5-fold after 28-day culture of cell-laden CSNI400 in the chondrogenic medium. These results suggest that the hybrid structure composed of the chitosan porous scaffold and the well-defined PNIPAAm hydrogel, in particular CSNI400, is suitable for 3D stem cell culture and cartilage tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2764-2774, 2016. |
Keywords: | 3D cell culture cartilage tissue engineering hybrid scaffold thermoresponsive hydrogel |
Rights: | © 2016 WILEY PERIODICALS, INC. |
DOI: | 10.1002/jbm.a.35810 |
Grant ID: | http://purl.org/au-research/grants/arc/DP160104632 |
Published version: | http://dx.doi.org/10.1002/jbm.a.35810 |
Appears in Collections: | Aurora harvest 3 Chemical Engineering publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.