Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/109311
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Validation of a rapid one-step high sensitivity real-time quantitative PCR system for detecting major BCR-ABL1 mRNA on an International Scale |
Author: | Yoshida, C. Nakamae, H. Fletcher, L. Koga, D. Sogabe, T. Matsumura, I. Kanakura, Y. Branford, S. Naoe, T. |
Citation: | SpringerPlus, 2016; 5(1):569-1-569-7 |
Publisher: | Springer |
Issue Date: | 2016 |
ISSN: | 2193-1801 2193-1801 |
Statement of Responsibility: | Chikashi Yoshida, Hirohisa Nakamae, Linda Fletcher, Daisuke Koga, Takayuki Sogabe, Itaru Matsumura, Yuzuru Kanakura, Susan Branford and Tomoki Naoe |
Abstract: | Background: Detection and quantitation of BCR-ABL1 transcripts are crucial for managing patients with chronic myeloid leukemia (CML). Although real-time quantitative polymerase chain reaction (RT-qPCR) can be measured on an International Scale (IS), this has not become fully universal. By using a WHO international standard panel established for calibrating secondary standards based on the IS, we have previously developed an RT-qPCR kit, ODK-1201, for quantification of major BCR-ABL1. Results: In this study, the reliability of kit-specific conversion factor 1.12 was validated by exchanging patients’ samples between three local clinical laboratories and a reference laboratory. The mean bias of the local method after IS conversion was 1.6 fold lower than the reference method. The clinically-useful sensitivity of the kit was further evaluated for monitoring patients with deep molecular response. Based on the correlation of the IS values between ODK-1201 and the reference laboratory method, the detection level of the kit was estimated as 0.0032 % BCR-ABL1 IS. Conclusions: ODK-1201 is a highly sensitive one-step RT-qPCR system for detecting BCR-ABL1 on the IS in 2 h after RNA extraction, thus contributing to standardization of molecular monitoring in CML. |
Keywords: | BCR-ABL1 Chronic myeloid leukemia Conversion factor International Scale Real-time quantitative PCR |
Rights: | © The Author(s). 2016 |
DOI: | 10.1186/s40064-016-2258-6 |
Published version: | http://dx.doi.org/10.1186/s40064-016-2258-6 |
Appears in Collections: | Aurora harvest 8 Medicine publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.