Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/111463
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dc.contributor.authorSatiaputra, J.en
dc.contributor.authorEijkelkamp, B.en
dc.contributor.authorMcDevitt, C.en
dc.contributor.authorShearwin, K.en
dc.contributor.authorBooker, G.en
dc.contributor.authorPolyak, S.en
dc.date.issued2018en
dc.identifier.citationApplied Microbiology and Biotechnology, 2018; 102(8):3793-3803en
dc.identifier.issn0175-7598en
dc.identifier.issn1432-0614en
dc.identifier.urihttp://hdl.handle.net/2440/111463-
dc.descriptionPublished online: 5 March 2018en
dc.description.abstractBiotin (Vitamin B7) is a critical enzyme co-factor in metabolic pathways important for bacterial survival. Biotin is obtained either from the environment or by de novo synthesis, with some bacteria capable of both. In certain species, the bifunctional protein BirA plays a key role in biotin homeostasis as it regulates expression of biotin biosynthetic enzymes in response to biotin demand and supply. Here, we compare the effect of biotin on the growth of two bacteria that possess a bifunctional BirA, namely Escherichia coli and Staphylococcus aureus. Unlike E. coli that could fulfill its biotin requirements through de novo synthesis, S. aureus showed improved growth rates in media supplemented with 10 nM biotin. S. aureus also accumulated more radiolabeled biotin from the media highlighting its ability to efficiently scavenge exogenous material. These data are consistent with S. aureus colonizing low biotin microhabitats. We also demonstrate that the S. aureus BirA protein is a transcriptional repressor of BioY, a subunit of the biotin transporter, and an operon containing yhfT and yhfS, the products of which have a putative role in fatty acid homeostasis. Increased expression of bioY is proposed to help cue S. aureus for efficient scavenging in low biotin environments.en
dc.description.statementofresponsibilityJiulia Satiaputra, Bart A. Eijkelkamp, Christopher A. McDevitt, Keith E. Shearwin, Grant W. Booker, Steven W. Polyaken
dc.language.isoenen
dc.publisherSpringer Verlagen
dc.rights© Springer-Verlag GmbH Germany, part of Springer Nature 2018en
dc.subjectBiotin; Gene expression/regulation; Staphylococcus aureus; BirA; Biotin protein ligaseen
dc.titleBiotin-mediated growth and gene expression in Staphylococcus aureus is highly responsive to environmental biotinen
dc.typeJournal articleen
dc.identifier.rmid0030083636en
dc.identifier.doi10.1007/s00253-018-8866-zen
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1068885en
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1080784en
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1122582en
dc.relation.granthttp://purl.org/au-research/grants/arc/DP160101450en
dc.relation.granthttp://purl.org/au-research/grants/arc/DP150101856en
dc.relation.granthttp://purl.org/au-research/grants/arc/DP170102102en
dc.identifier.pubid399729-
pubs.library.collectionMolecular and Biomedical Science publicationsen
pubs.library.teamDS03en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidEijkelkamp, B. [0000-0003-0179-8977]en
dc.identifier.orcidMcDevitt, C. [0000-0003-1596-4841]en
dc.identifier.orcidShearwin, K. [0000-0002-7736-2742]en
dc.identifier.orcidBooker, G. [0000-0001-7207-4699]en
dc.identifier.orcidPolyak, S. [0000-0002-8458-5194]en
Appears in Collections:Molecular and Biomedical Science publications

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