Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Transcriptional Sygernism Between Vitamin D-Rsponsive Elements in the Rat 25-Hyroxyvitamin D-3 24-Hydroxylase (CYP24) Promotor|
|Citation:||Journal of Biological Chemistry, 1996; 271(47):29715-29721|
|Publisher:||AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC|
|Abstract:||Transcription of the CYP24 gene is induced by 1,25-(OH)2D3 through a vitamin D receptor-dependent process. The functional activities of three possible vitamin D response elements (VDREs), located on the antisense strand of the rat CYP24 promoter, were investigated by transient expression of native and mutant promoter constructs in COS-1, JTC-12, and ROS 17/2.8 cells. A putative VDRE with a half-site spacing of 6 base pairs at -249/-232 (VDRE-3) did not contribute to 1,25-(OH)2D3 induced expression in the native promoter, although activity has been reported when the element was fused to the heterologous thymidine kinase promoter. Two VDREs with half-site spacings of 3 base pairs at -150/-136 and -258/-244 (VDRE-1 and VDRE-2, respectively), showed transcriptional synergism in COS-1 cells when treated with 1,25-(OH)2D3 (10(-7) to 10(-11) M). The contribution of both VDREs was hormone-concentration dependent from 10(-10) to 10(-12) M, with VDRE-1 demonstrating greatest sensitivity to 1,25-(OH)2D3. Transactivation by VDRE-1 was always greater than VDRE-2, but the converse was observed for the binding of vitamin D receptor-retinoid X receptor complex by each VDRE in gel mobility shift assays. The synergy observed between VDRE-1 and VDRE-2 may have important implications in cellular responses to different circulating levels of 1,25-(OH)2D3.|
|Keywords:||Cell Line; Animals; Rats; Cytochrome P-450 Enzyme System; Steroid Hydroxylases; Vitamin D; Nuclear Proteins; Mutagenesis, Site-Directed; Transcription, Genetic; Gene Expression Regulation, Enzymologic; Protein Binding; Promoter Regions, Genetic; Vitamin D3 24-Hydroxylase|
|Appears in Collections:||Biochemistry publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.