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https://hdl.handle.net/2440/11519
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Type: | Journal article |
Title: | Enhancement or inhibition of HIV-1 replication by intracellular expression of sense or antisense RNA targetted at different intermediates of reverse transcription. |
Author: | Peng, H. Callison, D. Li, P. Burrell, C. |
Citation: | AIDS, 1997; 11(5):587-595 |
Publisher: | LIPPINCOTT WILLIAMS & WILKINS |
Issue Date: | 1997 |
ISSN: | 0269-9370 1473-5571 |
Statement of Responsibility: | Hairong Peng, Deborah E. Callison, Peng Li and Christopher J. Burrell |
Abstract: | Objectives: To construct retroviral vectors expressing sense or antisense RNA targeted at HIV reverse transcription intermediates, and to test the anti-HIV properties of these constructs in transduced T cells. Design: Five double-copy retroviral vectors were constructed, in which the expression of the sense or antisense RNA corresponding to HIV minus- or plus-strand strong-stop DNA was driven by the human tRNAmet promoter. Method: The templates for the sense or antisense RNA were polymerase chain reaction-cloned from HIV pNL43 into a murine leukaemia virus-based vector and corresponding defective virions were packaged in PA317 cells. Human Jurkat T cells transduced with these vectors were challenged with HIV and monitored for viral RNA, viral DNA and p24 production for 23 weeks. Results: Intracellular expression of HIV sense RU5 sequences (RNA complementary to minus-strand strong-stop DNA) enhanced HIV replication in T cells. Expression of HIV sense or antisense U3RU5 sequences (identical or complementary to plus-strand strong-stop DNA) conferred long-term inhibition of HIV replication, despite continuous presence of viral challenge in the transduced cell cultures. Conclusion: Plus-strand strong-stop DNA as an intermediate in the early process of viral reverse transcription can be explored as an additional target for anti-HIV gene therapy. |
Keywords: | Reverse transcription retroviral vector antisense RNA gene therapy |
Rights: | Copyright: © Lippincott-Raven Publishers. |
DOI: | 10.1097/00002030-199705000-00006 |
Published version: | http://dx.doi.org/10.1097/00002030-199705000-00006 |
Appears in Collections: | Aurora harvest 7 Microbiology and Immunology publications |
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