Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/117712
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Tuning microenvironment for multicellular spheroid formation in thermo-responsive anionic microgel scaffolds
Author: Cui, X.
Hartanto, Y.
Wu, C.
Bi, J.
Dai, S.
Zhang, H.
Citation: Journal of Biomedical Materials Research - Part A, 2018; 106(11):2899-2909
Publisher: Wiley
Issue Date: 2018
ISSN: 1549-3296
1552-4965
Statement of
Responsibility: 
Xiaolin Cui, Yusak Hartanto, Chengtie Wu, Jingxiu Bi, Sheng Dai, Hu Zhang
Abstract: Tumor spheroids are considered to be effective in drug screening and evaluation. Three-dimensional scaffold-based cell culture becomes very promising in producing multicellular spheroids. Different from other approaches, 3D scaffolds mimic in vivo cellular microenvironment which encourages intercellular and extracellular interactions. The properties of the cellular microenvironment include the surface wettability, chemistry, and charge of the scaffolds which may influence cell attachment, proliferation as well as migration and these properties are essential for multicellular spheroids formation. Through co-polymerization with different carboxylic acids, we demonstrate that the surface charge density and hydrophobicity of the microenvironment have a great impact on the tumor spheroids formation progress and their size distribution. Our results show that a scaffold with a moderate negative charge density and a highly hydrophilic surface promotes cell proliferation, resulting in quicker and larger spheroids formation. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2899-2909, 2018.
Keywords: 3D cell culture; P(NIPAM); biomaterials; multicellular spheroids; thermosensitive microgels
Rights: © 2018 WILEY PERIODICALS, INC.
RMID: 0030102506
DOI: 10.1002/jbm.a.36479
Grant ID: http://purl.org/au-research/grants/arc/DP160104632
Appears in Collections:Chemical Engineering publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.