Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/119759
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Type: Journal article
Title: Development and characterization of primary cultures of smooth muscle cells from the fibromuscular stroma of the guinea pig prostate
Author: Ricciardelli, C.
Horsfall, D.
Skinner, J.
Henderson, D.
Marshall, V.
Tilley, W.
Citation: In Vitro Cellular & Developmental Biology, 1989; 25(11):1016-1024
Publisher: The Society for In Vitro Biology
Issue Date: 1989
ISSN: 0883-8364
1475-2689
Statement of
Responsibility: 
Carmela Ricciardelli, David J. Horsfall, John M. Skinner, Douglas W. Henderson, Villis R. Marshall, Wayne D. Tilley
Abstract: Primary cultures of smooth muscle cells (SMCs) were obtained by a two-step enzymatic digestion of guinea pig prostatic stroma. Ultrastructural morphology and growth characteristics of these cells conformed to those reported for SMCs isolated from vascular and visceral tissue sources. Electron microscopic examination indicated that the cells assumed modified myofibroblastoid features in culture. Microfilaments with associated dense bodies were markedly depleted in cultured smooth muscle cells, in comparison with those of the parent tissue. Cultured cells also possessed increased content of rough endoplasmic reticulum indicating the increased secretory or protein-synthetic capacity of the cells. Immunoperoxidase staining for cytoskeletal markers using monoclonal antibodies to desmin and vimentin supported the ultrastructural observations, suggesting a decline in desmin-staining intermediate filaments during "modulation" to the myofibroblastoid form. Despite this depletion of smooth muscle-specific differentiation markers and reversion to more general mesenchymal properties, the cells retained the ability to contract on challenge with norepinephrine, and grew in the characteristic "hill and valley" pattern on attaining confluence. Inasmuch as the estrogen and androgen receptor expression of the parent stromal tissue is also retained, these primary cell cultures should provide a useful model to study regulation of prostatic development.
Keywords: Smooth muscle cells; primary culture; prostate stroma; ultrastructure; differentiation; steroid receptors
Rights: © Tissue Culture Association, Inc 1989.
RMID: 0030062730
DOI: 10.1007/BF02624135
Appears in Collections:Medicine publications

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