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https://hdl.handle.net/2440/133290
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Type: | Journal article |
Title: | Spatial and temporal control of CRISPR-Cas9-mediated gene editing delivered via a light-triggered liposome system |
Author: | Aksoy, Y.A. Yang, B. Chen, W. Hung, T. Kuchel, R.P. Zammit, N.W. Grey, S.T. Goldys, E.M. Deng, W. |
Citation: | ACS Applied Materials and Interfaces, 2020; 12(47):52433-52444 |
Publisher: | American Chemical Society (ACS) |
Issue Date: | 2020 |
ISSN: | 1944-8244 1944-8252 |
Statement of Responsibility: | Yagiz Alp Aksoy, Biyao Yang, Wenjie Chen, Tzongtyng Hung, Rhiannon P. Kuchel, Nathan W. Zammit, Shane T. Grey, Ewa M. Goldys, and Wei Deng |
Abstract: | The CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with the CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with the CRISPR-Cas9 system. We demonstrated its efficient protein release by respectively assessing the targeted knockout of the eGFP gene in human HEK293/GFP cells and the TNFAIP3 gene in TNFα-induced HEK293 cells. We further validated our results at a single-cell resolution using an in vivo eGFP reporter system in zebrafish (77% knockout). These findings indicate that light-triggered liposomes may have new options for precise control of CRISPR-Cas9 release and editing. |
Keywords: | CRISPR-Cas9; liposomes; light triggering; temporal control; protein release |
Rights: | © 2020 American Chemical Society |
DOI: | 10.1021/acsami.0c16380 |
Grant ID: | http://purl.org/au-research/grants/arc/CE140100003 http://purl.org/au-research/grants/arc/DP170101863 |
Published version: | http://dx.doi.org/10.1021/acsami.0c16380 |
Appears in Collections: | Medicine publications |
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