Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/17128
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Severity-dependent expression of pro-inflammatory cytokines in traumatic spinal cord injury in the rat |
Author: | Yang, L. Jones, N. Blumbergs, P. Van Den Heuvel, C. Bradford, E. Manavis, J. Sarvestani, G. Ghabriel, M. |
Citation: | Journal of Clinical Neuroscience, 2005; 12(3):276-284 |
Publisher: | Churchill Livingstone |
Issue Date: | 2005 |
ISSN: | 0967-5868 1532-2653 |
Statement of Responsibility: | Liqun Yang, Nigel R. Jones, Peter C. Blumbergs, Corinna Van Den Heuvel, Emma J. Moore, Jim Manavis, Ghafar T. Sarvestani and Mounir N. Ghabriel |
Abstract: | The post-traumatic inflammatory response in acute spinal cord contusion injury was studied in the rat. Mild and severe spinal cord injury (SCI) was produced by dropping a 10 g weight from 3 and 12 cm at the T12 vertebral level. Increased immunoreactivity of TNF-alpha in mild and severe SCI was detected in neurons at 1 h post-injury, and in neurons and microglia at 6 h post-injury, with a less significant increase in mild SCI. Expression was short-lived and declined sharply by 1 d post-injury. RT-PCR showed an early significant up-regulation of IL-1 beta, IL-6 and TNF-alpha mRNAs, maximal at 6 h post-injury with return to control levels by 24 h post-injury, the changes being less statistically significantly in mild SCI. Western blot showed early transient increases of IL-1 beta, IL-6 and TNF-alpha proteins in severe SCI but not mild SCI. Immunocytochemical, western blotting and RT-PCR analyses suggest that endogenous cells (neurons and microglia) in the spinal cord, not blood-borne leucocytes, contribute to IL-1 beta, IL-6 and TNF-alpha production in the post-traumatic inflammatory response and that their up-regulation is greater in severe than mild SCI. |
Keywords: | interleukin-1β interleukin-6 tumour necrosis factor-* traumatic spinal cord injury microglia inflammatory response |
Description: | Copyright © 2005 Elsevier Ltd All rights reserved. |
DOI: | 10.1016/j.jocn.2004.06.011 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/623056/description#description |
Published version: | http://dx.doi.org/10.1016/j.jocn.2004.06.011 |
Appears in Collections: | Anatomical Sciences publications Aurora harvest 6 |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.