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|Title:||Severity-dependent expression of pro-inflammatory cytokines in traumatic spinal cord injury in the rat|
Van Den Heuvel, C.
|Citation:||Journal of Clinical Neuroscience, 2005; 12(3):276-284|
|Liqun Yang, Nigel R. Jones, Peter C. Blumbergs, Corinna Van Den Heuvel, Emma J. Moore, Jim Manavis, Ghafar T. Sarvestani and Mounir N. Ghabriel|
|Abstract:||The post-traumatic inflammatory response in acute spinal cord contusion injury was studied in the rat. Mild and severe spinal cord injury (SCI) was produced by dropping a 10 g weight from 3 and 12 cm at the T12 vertebral level. Increased immunoreactivity of TNF-alpha in mild and severe SCI was detected in neurons at 1 h post-injury, and in neurons and microglia at 6 h post-injury, with a less significant increase in mild SCI. Expression was short-lived and declined sharply by 1 d post-injury. RT-PCR showed an early significant up-regulation of IL-1 beta, IL-6 and TNF-alpha mRNAs, maximal at 6 h post-injury with return to control levels by 24 h post-injury, the changes being less statistically significantly in mild SCI. Western blot showed early transient increases of IL-1 beta, IL-6 and TNF-alpha proteins in severe SCI but not mild SCI. Immunocytochemical, western blotting and RT-PCR analyses suggest that endogenous cells (neurons and microglia) in the spinal cord, not blood-borne leucocytes, contribute to IL-1 beta, IL-6 and TNF-alpha production in the post-traumatic inflammatory response and that their up-regulation is greater in severe than mild SCI.|
|Keywords:||interleukin-1β; interleukin-6; tumour necrosis factor-*; traumatic spinal cord injury; microglia; inflammatory response|
|Description:||Copyright © 2005 Elsevier Ltd All rights reserved.|
|Appears in Collections:||Anatomical Sciences publications|
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