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Type: Journal article
Title: An Arabidopsis callose synthase, GSL5, is required for wound and papillary callose formation
Author: Jacobs, A.
Lipka, V.
Burton, R.
Panstruga, R.
Strizhov, N.
Schulze-Lefert, P.
Fincher, G.
Citation: Plant Cell, 2003; 15(11):2503-2513
Publisher: Amer Soc Plant Physiologists
Issue Date: 2003
ISSN: 1040-4651
Statement of
Andrew K. Jacobs, Volker Lipka, Rachel A. Burton, Ralph Panstruga, Nicolai Strizhov, Paul Schulze-Lefert and Geoffrey B. Fincher
Abstract: Arabidopsis was transformed with double-stranded RNA interference (dsRNAi) constructs designed to silence three putative callose synthase genes: GLUCAN SYNTHASE–LIKE5 (GSL5), GSL6, and GSL11. Both wound callose and papillary callose were absent in lines transformed with GSL5 dsRNAi and in a corresponding sequence-indexed GSL5 T-DNA insertion line but were unaffected in GSL6 and GSL11 dsRNAi lines. These data provide strong genetic evidence that the GSL genes of higher plants encode proteins that are essential for callose formation. Deposition of callosic plugs, or papillae, at sites of fungal penetration is a widely recognized early response of host plants to microbial attack and has been implicated in impeding entry of the fungus. Depletion of callose from papillae in gsl5 plants marginally enhanced the penetration of the grass powdery mildew fungus Blumeria graminis on the nonhost Arabidopsis. Paradoxically, the absence of callose in papillae or haustorial complexes correlated with the effective growth cessation of several normally virulent powdery mildew species and of Peronospora parasitica.
Keywords: Fungi; Plants, Genetically Modified; Arabidopsis; Plant Leaves; Glucans; Glucosyltransferases; Schizosaccharomyces pombe Proteins; Membrane Proteins; DNA, Bacterial; RNA, Double-Stranded; Signal Transduction; Plant Diseases; RNA Interference; Mutation; Stress, Mechanical; Immunity, Innate; Fungal Structures
RMID: 0020031004
DOI: 10.1105/tpc.016097
Appears in Collections:Agriculture, Food and Wine publications

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