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https://hdl.handle.net/2440/23361
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dc.contributor.author | Fotia, A. | - |
dc.contributor.author | Cook, D. | - |
dc.contributor.author | Kumar, S. | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | The International Journal of Biochemistry and Cell Biology, 2006; 38(3):472-479 | - |
dc.identifier.issn | 1357-2725 | - |
dc.identifier.issn | 1878-5875 | - |
dc.identifier.uri | http://hdl.handle.net/2440/23361 | - |
dc.description.abstract | Nedd4 and Nedd4-2 are closely related HECT-type ubiquitin-protein ligases (E3) implicated in the regulation of a number of proteins and pathways. Given the close homology between these E3 enzymes it would be predicted that a conserved ubiquitin-conjugating enzyme (E2) specificity exists between the two proteins. However, E2 specificities for Nedd4 and Nedd4-2 are not well established. In the present studies we aimed at clarifying the E2-specificities of Nedd4 and Nedd4-2 using in vitro ubiquitination assays. We demonstrate strong substrate ubiquitination in the presence of UbcH5b by both Nedd4 and Nedd4-2. We also found that Ube2e3, an E2 previously shown to be used by Nedd4-2, is used less efficiently than UbcH5b. Our results suggest that for optimal ubiquitination Nedd4 and Nedd4-2 require the same E2 enzymes. | - |
dc.language.iso | en | - |
dc.publisher | Pergamon-Elsevier Science Ltd | - |
dc.source.uri | http://dx.doi.org/10.1016/j.biocel.2005.11.006 | - |
dc.subject | E2 | - |
dc.subject | E3 | - |
dc.subject | ubiquitination | - |
dc.subject | ubiquitin-protein ligase | - |
dc.subject | ubiquitin-conjugating enzyme | - |
dc.subject | sodium channel | - |
dc.subject | substrates | - |
dc.subject | N4BP1 | - |
dc.title | The ubiquitin-protein ligases Nedd4 and Nedd4-2 show similar ubiquitin-conjugating enzyme specificities | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.biocel.2005.11.006 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Kumar, S. [0000-0001-7126-9814] | - |
Appears in Collections: | Aurora harvest 2 Medicine publications |
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