Adelaide Research & Scholarship
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https://hdl.handle.net/2440/46933
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| Type: | Journal article |
| Title: | Systematic screening of sheep skin cDNA libraries for microsatellite sequences |
| Author: | DeSilva, U. Franklin, I. Maddox, J. van Hest, B. Adelson, D. |
| Citation: | Cytogenetic and Genome Research, 2003; 102(1-4):79-84 |
| Publisher: | Karger |
| Issue Date: | 2003 |
| ISSN: | 1424-8581 1424-859X |
| Statement of Responsibility: | U. DeSilva, I.R. Franklin, J.F. Maddox, B. van Hest, D.L. Adelson |
| Abstract: | 65,000 sheep skin cDNA clones were gridded in high density on to nylon membranes and screened for (CA)n and (GA)n repeat containing clones. 296 dinucleotide repeat-containing clones were identified with ~85% non-redundancy. Clones were single-pass 5' sequenced and we compared the Expressed Sequence Tag (EST) sequences to the Swiss-Prot database to ascertain their identity and/or putative function. We then aligned the ESTs against the human genomic sequence to determine the locations of human orthologous sequences. Finally, we developed a subset of polymorphic microsatellite markers and positioned them on the ovine linkage map. |
| Keywords: | Skin Animals Sheep, Domestic Humans DNA, Complementary Chromosome Mapping Cloning, Molecular Microsatellite Repeats Dinucleotide Repeats Gene Library Genome, Human Expressed Sequence Tags Databases, Genetic |
| Description: | © 2008 S. Karger AG, Basel |
| DOI: | 10.1159/000075729 |
| Published version: | http://dx.doi.org/10.1159/000075729 |
| Appears in Collections: | Aurora harvest 6 Environment Institute publications Molecular and Biomedical Science publications |
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