Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/54811
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Type: Journal article
Title: Degradation of Supercoiled Plasmid DNA Within a Capillary Device
Author: Meacle, F.
Zhang, H.
Papantoniou, I.
Ward, J.
Titchener-Hooker, N.
Hoare, M.
Citation: Biotechnology and Bioengineering, 2007; 97(5):1148-1157
Publisher: John Wiley & Sons Inc
Issue Date: 2007
ISSN: 0006-3592
1097-0290
Statement of
Responsibility: 
F.J. Meacle, H. Zhang, I. Papantoniou, J.M. Ward, N.J. Titchener-Hooker and M. Hoare
Abstract: Supercoiled plasmid DNA is susceptible to fluid stress in large-scale manufacturing processes. A capillary device was used to generate controlled shear conditions and the effects of different stresses on plasmid DNA structure were investigated. Computational fluid dynamics (CFD) analysis was employed to characterize the flow environment in the capillary device and different analytical techniques were used to quantify the DNA breakage. It was found that the degradation of plasmid DNA occurred at the entrance of the capillary and that the shear stress within the capillary did not affect the DNA structure. The degradation rate of plasmids was well correlated with the average elongational strain rate or the pressure drop at the entrance region. The conclusion may also be drawn that laminar shear stress does not play a significant role in plasmid DNA degradation.
Keywords: plasmid DNA; capillary device; shear rate; breakage; computational fluid dynamics
RMID: 0020093538
DOI: 10.1002/bit.21275
Appears in Collections:Chemical Engineering publications

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