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Type: Journal article
Title: Pro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositis
Author: Ong, Z.
Gibson, R.
Bowen, J.
Stringer, A.
Darby, J.
Logan, R.
Yeoh, A.
Keefe, D.
Citation: Radiation Oncology, 2010; 5(22):1-8
Publisher: BioMed Central Ltd.
Issue Date: 2010
ISSN: 1748-717X
Statement of
Zhi Yi Ong, Rachel J. Gibson, Joanne M. Bowen, Andrea M. Stringer, Jocelyn M. Darby, Richard M. Logan, Ann S.J. Yeoh, Dorothy M. Keefe
Abstract: Background: Mucositis is a toxic side effect of anti-cancer treatments and is a major focus in cancer research. Pro-inflammatory cytokines have previously been implicated in the pathophysiology of chemotherapy-induced gastrointestinal mucositis. However, whether they play a key role in the development of radiotherapy-induced gastrointestinal mucositis is still unknown. Therefore, the aim of the present study was to characterise the expression of pro-inflammatory cytokines in the gastrointestinal tract using a rat model of fractionated radiotherapy-induced toxicity. Methods: Thirty six female Dark Agouti rats were randomly assigned into groups and received 2.5 Gys abdominal radiotherapy three times a week over six weeks. Real time PCR was conducted to determine the relative change in mRNA expression of pro-inflammatory cytokines IL-1β, IL-6 and TNF in the jejunum and colon. Protein expression of IL-1β, IL-6 and TNF in the intestinal epithelium was investigated using qualitative immunohistochemistry. Results: Radiotherapy-induced sub-acute damage was associated with significantly upregulated IL-1β, IL-6 and TNF mRNA levels in the jejunum and colon. The majority of pro-inflammatory cytokine protein expression in the jejunum and colon exhibited minimal change following fractionated radiotherapy. Conclusions: Pro-inflammatory cytokines play a key role in radiotherapy-induced gastrointestinal mucositis in the sub-acute onset setting.
Keywords: Intestinal Mucosa; Animals; Rats; Inflammation; RNA, Messenger; Cytokines; Immunohistochemistry; Reverse Transcriptase Polymerase Chain Reaction; Female; Radiotherapy, Intensity-Modulated; Mucositis
Rights: © 2010 Ong et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0020096240
DOI: 10.1186/1748-717X-5-22
Appears in Collections:Medicine publications

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