Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/59751
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Type: Journal article
Title: Pro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositis
Author: Ong, Z.
Gibson, R.
Bowen, J.
Stringer, A.
Darby, J.
Logan, R.
Yeoh, A.
Keefe, D.
Citation: Radiation Oncology, 2010; 5(22):1-8
Publisher: BioMed Central Ltd.
Issue Date: 2010
ISSN: 1748-717X
1748-717X
Statement of
Responsibility: 
Zhi Yi Ong, Rachel J. Gibson, Joanne M. Bowen, Andrea M. Stringer, Jocelyn M. Darby, Richard M. Logan, Ann S.J. Yeoh, Dorothy M. Keefe
Abstract: Background: Mucositis is a toxic side effect of anti-cancer treatments and is a major focus in cancer research. Pro-inflammatory cytokines have previously been implicated in the pathophysiology of chemotherapy-induced gastrointestinal mucositis. However, whether they play a key role in the development of radiotherapy-induced gastrointestinal mucositis is still unknown. Therefore, the aim of the present study was to characterise the expression of pro-inflammatory cytokines in the gastrointestinal tract using a rat model of fractionated radiotherapy-induced toxicity. Methods: Thirty six female Dark Agouti rats were randomly assigned into groups and received 2.5 Gys abdominal radiotherapy three times a week over six weeks. Real time PCR was conducted to determine the relative change in mRNA expression of pro-inflammatory cytokines IL-1β, IL-6 and TNF in the jejunum and colon. Protein expression of IL-1β, IL-6 and TNF in the intestinal epithelium was investigated using qualitative immunohistochemistry. Results: Radiotherapy-induced sub-acute damage was associated with significantly upregulated IL-1β, IL-6 and TNF mRNA levels in the jejunum and colon. The majority of pro-inflammatory cytokine protein expression in the jejunum and colon exhibited minimal change following fractionated radiotherapy. Conclusions: Pro-inflammatory cytokines play a key role in radiotherapy-induced gastrointestinal mucositis in the sub-acute onset setting.
Keywords: Intestinal Mucosa; Animals; Rats; Inflammation; RNA, Messenger; Cytokines; Immunohistochemistry; Reverse Transcriptase Polymerase Chain Reaction; Female; Radiotherapy, Intensity-Modulated; Mucositis
Rights: © 2010 Ong et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0020096240
DOI: 10.1186/1748-717X-5-22
Appears in Collections:Medicine publications

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