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https://hdl.handle.net/2440/59936
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Type: | Journal article |
Title: | Transfer rate measurement of lysozyme by liquid-liquid extraction using reverse micelles with dense CO2 |
Author: | Shin, U. Jung, S. Uddin, M. Park, S. Kishimura, H. Wilkinson, G. Chun, B. |
Citation: | Korean Journal of Chemical Engineering, 2010; 27(2):596-601 |
Publisher: | Korean Inst Chem Engineers |
Issue Date: | 2010 |
ISSN: | 0256-1115 1975-7220 |
Statement of Responsibility: | Sun-Mi Jung, Un-Mi Shin, Md. Salim Uddin, Sun-Young Park, Hideki Kishimura, Gordon Wilkinson and Byung-Soo Chun |
Abstract: | Lysozyme was extracted from aqueous solution into i-octane using reverse micelles in the presence of pressurized CO2. A squat vessel with two independent stirrers was used to measure the mass transfer of the lysozyme across a planar interface. Mass transfer coefficient, k L of the lysozyme from the aqueous to the organic phase was measured at selected ionic strengths, pH, sodium bis(2-ethylhexyl) sulfosuccinate (AOT) surfactant concentrations, temperatures and pressurized CO2. The mass transfer rate of lysozyme was higher in high temperature (318 K) and pressure (20MPa). pH of 9 in aqueous phase showed highest mass transfer rate of lysozyme. The application of pressurized CO2 markedly increased the mass transfer rate of lysozyme comparing to conventional non-pressurized system. |
Keywords: | Lysozyme Reverse Micelle Mass Transfer Pressurized CO2 Sodium Bis(2-ethylhexyl) Sulfosuccinate (AOT) |
Rights: | © 2010 Springer, Part of Springer Science+Business Media |
DOI: | 10.2478/s11814-010-0063-4 |
Published version: | http://www.springerlink.com/content/fkj8017k7125587k/ |
Appears in Collections: | Aurora harvest Chemical Engineering publications |
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