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https://hdl.handle.net/2440/66412
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Type: | Journal article |
Title: | Enhanced molecular chaperone activity of the small heat-shock protein αβ-crystallin following covalent immobilization onto a solid-phase support |
Other Titles: | Enhanced molecular chaperone activity of the small heat-shock protein alpha beta-crystallin following covalent immobilization onto a solid-phase support |
Author: | Garvey, M. Griesser, S. Griesser, H. Thierry, B. Nussio, M. Shapter, J. Ecroyd, H. Giorgetti, S. Bellotti, V. Gerrard, J. Carver, J. |
Citation: | Biopolymers, 2011; 95(6):376-389 |
Publisher: | John Wiley & Sons Inc |
Issue Date: | 2011 |
ISSN: | 0006-3525 1097-0282 |
Statement of Responsibility: | Megan Garvey, Stefani S. Griesser, Hans J. Griesser, Benjamin Thierry, Matthew R. Nussio, Joseph G. Shapter, Heath Ecroyd, Sofia Giorgetti, Vittorio Bellotti, Juliet A. Gerrard and John A. Carver |
Abstract: | We report on an improved method to interpret single molecule dissociation measurements using atomic force microscopy. We describe an easy to use methodology to reject nonspecific binding events, as well as estimating the number of multiple binding events. The method takes nonlinearities in the force profiles into account that result from the deformation of the used polymeric linkers. This new method is applied to a relevant enzyme−inhibitor system, latent matrix metalloprotease 9 (ProMMP-9, a gelatinase), and its inhibitor, tissue inhibitor of metalloproteases 1 (TIMP 1), which are important players in cancer metastasis. Our method provides a measured kinetic off-rate of 0.010 ± 0.003 s−1 for the dissociation of ProMMP9 and TIMP1, which is consistent with values measured by ensemble methods. |
Keywords: | Amyloid Macroglobulins Caseins alpha-Crystallin B Chain Molecular Chaperones Solutions Microscopy, Atomic Force Protein Binding Protein Folding Surface Properties Heat-Shock Proteins, Small Immobilized Proteins Photoelectron Spectroscopy Quartz Crystal Microbalance Techniques |
Rights: | Copyright © 2011 Wiley Periodicals, Inc. |
DOI: | 10.1002/bip.21584 |
Grant ID: | ARC |
Published version: | http://dx.doi.org/10.1002/bip.21584 |
Appears in Collections: | Aurora harvest 5 IPAS publications |
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