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Type: Journal article
Title: Centrifugal recovery and dissolution of recombinant Gly-IGF-II inclusion bodies: the impact of feedrate and recentrifugation on protein yield
Author: Wong, H.
O'Neill, B.
Middelberg, A.
Citation: Bioseparation, 1996; 6(3):185-192
Issue Date: 1996
ISSN: 0923-179X
Abstract: The impact of centrifuge feedrate and multiple centrifuge passes on protein yield following recombinant Gly-Insulin-like Growth Factor II (Gly-IGF-II) inclusion-body dissolution has been investigated. Altering centrifuge feedrate did not significantly improve the overall protein yield following dissolution. Improved centrifuge recovery at a low feedrate was offset by poorer inclusion body paste purity. This reduced purity resulted in a significant loss of protein during inclusion-body dissolution due to proteolysis. Multiple centrifuge passes improved the inclusion-body paste purity. This resulted in a net improvement in the overall protein yield following dissolution. This work demonstrates that a strong interaction exists between centrifuge performance and inclusion-body dissolution for protease-sensitive products such as Gly-IGF-II.
Keywords: Inclusion Bodies
Escherichia coli
Insulin-Like Growth Factor II
Recombinant Proteins
Evaluation Studies as Topic
Appears in Collections:Aurora harvest 6
Chemical Engineering publications

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