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https://hdl.handle.net/2440/78386
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Type: | Journal article |
Title: | Matrix-assisted laser desorption/ionization imaging protocol for in situ characterization of tryptic peptide identity and distribution in formalin-fixed tissue |
Author: | Gustafsson, O. Eddes, J. Meding, S. McColl, S. Oehler, M. Hoffmann, P. |
Citation: | Rapid Communications in Mass Spectrometry, 2013; 27(6):655-670 |
Publisher: | John Wiley & Sons Ltd |
Issue Date: | 2013 |
ISSN: | 0951-4198 1097-0231 |
Statement of Responsibility: | Ove J. R. Gustafsson, James S. Eddes, Stephan Meding, Shaun R. McColl, Martin K. Oehler and Peter Hoffmann |
Abstract: | <h4>Rationale</h4>Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry provides the means to map the in situ distribution of tryptic peptides in formalin-fixed clinical tissue samples. The ability to analyze clinical samples is of great importance to further developments in the imaging field. However, there is a requirement in this field of research for additional methods describing the characterization of tryptic peptides by MALDI imaging.<h4>Methods and results</h4>This protocol gives highly detailed instructions, with examples, for (1) successfully performing tryptic peptide MALDI imaging on formalin-fixed cancer tissue using a MALDI-TOF/TOF MS instrument, (2) tentatively generating identifications through nLC/MS/MS, and (3) validating these identifications by in situ MS/MS of peptides of interest.<h4>Conclusions</h4>This protocol provides a detailed and straightforward description of the methods required for groups new to MALDI imaging to begin analysis of formalin-fixed clinical samples. |
Keywords: | Humans Neoplasms Formaldehyde Trypsin Peptide Fragments Calibration Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Histocytological Preparation Techniques Amino Acid Sequence Image Processing, Computer-Assisted Molecular Sequence Data Tandem Mass Spectrometry Molecular Imaging |
Rights: | Copyright © 2013 John Wiley & Sons, Ltd. |
DOI: | 10.1002/rcm.6488 |
Grant ID: | http://purl.org/au-research/grants/arc/LP110100693 http://purl.org/au-research/grants/arc/LP110100693 |
Published version: | http://dx.doi.org/10.1002/rcm.6488 |
Appears in Collections: | Aurora harvest IPAS publications |
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