Adelaide Research & Scholarship
Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/790
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| Type: | Journal article |
| Title: | Correlation of O2 uptake rate and mitochondrial activity in carrot cell cultures exposed to laminar fluid shear |
| Author: | Wong, V. Williams, D. Colby, C. Saint, D. |
| Citation: | Biotechnology Letters, 2000; 22(24):1919-1924 |
| Publisher: | Kluwer Academic Publ |
| Issue Date: | 2000 |
| ISSN: | 0141-5492 1573-6776 |
| Abstract: | Measuring uncoupled oxygen uptake rate (OUR) could provide a convenient method for quantifying changes in the metabolic activity of plant cultures caused by hydrodynamic shear. Experiments on Daucus carota (carrot) cells were performed in a novel O2-permeable Couette viscometer at varying levels of laminar shear (6 to 100 N m-2). When the uncoupled OUR of the cells was compared with mitochondrial activity (determined by 2,3,5-triphenyl tetrazolium chloride assay), a significant correlation was observed (R = 0.91 by linear regression). |
| DOI: | 10.1023/A:1026752418909 |
| Published version: | http://dx.doi.org/10.1023/a:1026752418909 |
| Appears in Collections: | Aurora harvest 2 Chemical Engineering publications |
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