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https://hdl.handle.net/2440/79562
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dc.contributor.author | Wang, H. | - |
dc.contributor.author | Paton, J. | - |
dc.contributor.author | Herdman, B. | - |
dc.contributor.author | Rogers, T. | - |
dc.contributor.author | Beddoe, T. | - |
dc.contributor.author | Paton, A. | - |
dc.contributor.editor | Pirofski, L. | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Infection and Immunity, 2013; 81(3):673-683 | - |
dc.identifier.issn | 0019-9567 | - |
dc.identifier.issn | 1098-5522 | - |
dc.identifier.uri | http://hdl.handle.net/2440/79562 | - |
dc.description.abstract | The principal function of bacterial AB5 toxin B subunits is to interact with glycan receptors on the surfaces of target cells and mediate the internalization of holotoxin. However, B subunit-receptor interactions also have the potential to impact cell signaling pathways and, in so doing, contribute to pathogenesis independently of the catalytic (toxic) A subunits. Various Salmonella enterica serovars, including Salmonella enterica serovar Typhi, encode an AB5 toxin (ArtAB), the A subunit of which is an ADP-ribosyltransferase related to the S1 subunit of pertussis toxin. However, although the A subunit is able to catalyze ADP-ribosylation of host G proteins, a cytotoxic phenotype has yet to be identified for the holotoxin. We therefore examined the capacity of the purified B subunit (ArtB) from S. Typhi to elicit cytokine, chemokine, and adhesion molecule responses in human macrophage (U937), colonic epithelial (HCT-8) cell, and brain microvascular endothelial cell (HBMEC) lines. Secretion of the chemokines monocyte chemotactic protein 1 (MCP-1) and interleukin 8 (IL-8) was increased in all three tested cell lines, with macrophage inflammatory protein 1α (MIP-1α), MIP-1β, and granulocyte colony-stimulating factor (G-CSF) also significantly increased in U937 cells. ArtB also upregulated the cytokines tumor necrosis factor alpha (TNF-α) and IL-6 in HBMECs and HCT-8 cells, but not in U937 cells, while intercellular adhesion molecule 1 (ICAM-1) was upregulated in HCT-8 and U937 cells and vascular cell adhesion molecule 1 (VCAM-1) was upregulated in HBMECs. Thus, ArtB may contribute to pathogenesis independently of the A subunit by promoting and maintaining a strong inflammatory response at the site of infection. | - |
dc.description.statementofresponsibility | Hui Wang, James C. Paton, Brock P. Herdman, Trisha J. Rogers, Travis Beddoe, Adrienne W. Paton | - |
dc.language.iso | en | - |
dc.publisher | Amer Soc Microbiology | - |
dc.rights | Copyright © 2013, American Society for Microbiology. All Rights Reserved. | - |
dc.source.uri | http://dx.doi.org/10.1128/iai.01043-12 | - |
dc.subject | Intestinal Mucosa | - |
dc.subject | Brain | - |
dc.subject | Cell Line | - |
dc.subject | Macrophages | - |
dc.subject | Endothelial Cells | - |
dc.subject | Epithelial Cells | - |
dc.subject | Humans | - |
dc.subject | Salmonella typhi | - |
dc.subject | Protein Subunits | - |
dc.subject | Bacterial Toxins | - |
dc.subject | Cytokines | - |
dc.subject | Cell Adhesion | - |
dc.subject | Gene Expression Regulation | - |
dc.subject | Dose-Response Relationship, Drug | - |
dc.title | The b subunit of an AB5 toxin produced by salmonella enterica serovar typhi up-regulates chemokines, cytokines, and adhesion molecules in human macrophage, colonic epithelial, and brain microvascular endothelial cell lines | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1128/IAI.01043-12 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/DP1095420 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/DP120103178 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/DP1095420 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Paton, J. [0000-0001-9807-5278] | - |
Appears in Collections: | Aurora harvest 4 Molecular and Biomedical Science publications |
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