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Type: Journal article
Title: A mathematical model for Escherichia coli debris size reduction during high pressure homogenisation based on grinding theory
Author: Wong, H.
O'Neill, B.
Middelberg, A.
Citation: Chemical Engineering Science, 1997; 52(17):2883-2890
Issue Date: 1997
ISSN: 0009-2509
Abstract: Experimental data for E. coli debris size reduction during high-pressure homogenisation at 55 MPa are presented. A mathematical model based off grinding theory is developed to describe the data. The model is based on first-order breakage and compensation conditions. It does not require any assumption of a specified distribution for debris size and can be used given information on the initial size-distribution of whole cells and the disruption efficiency during homogenisation. The number of homogeniser passes is incorporated into the model and used to describe the size reduction of non-induced stationary and induced E. coli cells during homogenisation. Regressing the results to the model equations gave an excellent fit to experimental data (> 98.7% of variance explained for both fermentations), confirming the model's potential for predicting size reduction during high-pressure homogenisation. This study provides a means to optimise both homogenisation and disc-stack centrifugation conditions for recombinant product recovery.
DOI: 10.1016/S0009-2509(97)00105-X
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Chemical Engineering publications

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