Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/81806
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Type: Journal article
Title: Mapping of chromosomal loci associated with lipopolysaccharide synthesis and serotype specificity in Vibrio cholerae 01 by transposon mutagenesis using Tn5 and Tn2680
Author: Ward, H.
Manning, P.
Citation: Molecular and General Genetics, 1989; 218(2):367-370
Publisher: Springer Verlag
Issue Date: 1989
ISSN: 0026-8925
1432-1874
Statement of
Responsibility: 
Helena M. Ward and Paul A. Manning
Abstract: Vibrio cholerae strains of the 01 serotype have been classified into three subclasses, Ogawa, Inaba and Hikojima, which are associated with the O-antigen of the lipopolysaccharide (LPS). The DNA encoding the biosynthesis of the O-antigen, the rfb locus, has been cloned and analysed (Manning et al. 1986; Ward et al. 1987). Transposon mutagenesis of the Inaba and Ogawa strains of V. cholerae, using Tn5 or Tn2680 allowed the isolation of a series of independent mutants in each of these serotypes. Some of the insertions were mapped to the rfb region by Southern hybridization using the cloned rfb DNA as a probe, confirming this location to be responsible for both O-antigen production and serotype specificity. The other insertions allowed a second region to be identified which is involved in V. cholerae LPS biosynthesis.
Keywords: Bacteriophage resistance, O-antigen; Conjugation; LPS; P-factor
Rights: © Springer-Verlag 1989
RMID: 0030000213
DOI: 10.1007/BF00331294
Appears in Collections:Medical Education Unit publications

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