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https://hdl.handle.net/2440/8567
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Type: | Journal article |
Title: | Identification of septins in neurofibrillary tangles in Alzheimers Disease |
Author: | Kinoshita, A. Kinshita, M. Akiyama, H. Tomimoto, H. Akiguchi, I. Kumar, S. Noda, M. Kimura, J. |
Citation: | American Journal of Pathology, 1998; 153(5):1551-1560 |
Publisher: | AMER SOC INVESTIGATIVE PATHOLOGY, INC |
Issue Date: | 1998 |
ISSN: | 0002-9440 1525-2191 |
Statement of Responsibility: | Kinoshita, Ayae ; Kinoshita, Makoto ; Akiyama, Haruhiko ; Tomimoto, Hidekazu ; Akiguchi, Ichiro ; Kumar, Sharad ; Noda, Makoto ; Kimura, Jun |
Abstract: | Septins are evolutionarily conserved cytoskeletal GTPases that can form heteropolymer complexes involved in cytokinesis and other cellular processes. We detected expression of the human septin genes Nedd5, H5, Diff6, and hCDC100 in postmortem brain tissues using the reverse transcription-coupled polymerase chain reaction and their products by immunoblot analysis. Four antibodies directed against three septins, Nedd5, H5, and Diff6, consistently labeled neurofibrillary tangles, neuropil threads, and dystrophic neurites in the senile plaques in brains affected by Alzheimer's disease but did not label obvious structures in young control brains. Immunoelectron microscopy revealed that Nedd5 localized to the paired helical filaments. Pre-tangles, the precursory granular deposits that accumulate in the neuronal cytoplasm, also were labeled with the antibodies. These findings suggest that at least the three septins are associated with tau-based paired helical filament core, and may contribute to the formation of neurofibrillary tangle as integral constituents of paired helical filaments. |
Keywords: | Brain Neurofibrillary Tangles Humans Alzheimer Disease GTP Phosphohydrolases Phosphoric Monoester Hydrolases Cell Cycle Proteins Cytoskeletal Proteins tau Proteins Schizosaccharomyces pombe Proteins Transcription Factors Antibodies Microscopy, Immunoelectron Immunohistochemistry Brain Chemistry Molecular Sequence Data Adult Aged Aged, 80 and over Middle Aged Septins |
DOI: | 10.1016/S0002-9440(10)65743-4 |
Published version: | http://dx.doi.org/10.1016/s0002-9440(10)65743-4 |
Appears in Collections: | Aurora harvest 4 Medicine publications |
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