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https://hdl.handle.net/2440/88610
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dc.contributor.author | Lopaticki, S. | - |
dc.contributor.author | Maier, A. | - |
dc.contributor.author | Thompson, J. | - |
dc.contributor.author | Wilson, D. | - |
dc.contributor.author | Tham, W. | - |
dc.contributor.author | Triglia, T. | - |
dc.contributor.author | Gout, A. | - |
dc.contributor.author | Speed, T. | - |
dc.contributor.author | Beeson, J. | - |
dc.contributor.author | Healer, J. | - |
dc.contributor.author | Cowman, A. | - |
dc.contributor.editor | Adams, J.H. | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Infection and Immunity, 2011; 79(3):1107-1117 | - |
dc.identifier.issn | 0019-9567 | - |
dc.identifier.issn | 1098-5522 | - |
dc.identifier.uri | http://hdl.handle.net/2440/88610 | - |
dc.description.abstract | Plasmodium falciparum causes the most severe form of malaria in humans and invades erythrocytes using multiple ligand-receptor interactions. Two important protein families involved in erythrocyte binding are the erythrocyte binding-like (EBL) and the reticulocyte binding-like (RBL or P. falciparum Rh [PfRh]) proteins. We constructed P. falciparum lines lacking expression of EBL proteins by creating single and double knockouts of the corresponding genes for eba-175, eba-181, and eba-140 and show that the EBL and PfRh proteins function cooperatively, consistent with them playing a similar role in merozoite invasion. We provide evidence that PfRh and EBL proteins functionally interact, as loss of function of EBA-181 ablates the ability of PfRh2a/b protein antibodies to inhibit merozoite invasion. Additionally, loss of function of some ebl genes results in selection for increased transcription of the PfRh family. This provides a rational basis for considering PfRh and EBL proteins for use as a combination vaccine against P. falciparum. We immunized rabbits with combinations of PfRh and EBL proteins to test the ability of antibodies to block merozoite invasion in growth inhibition assays. A combination of EBA-175, PfRh2a/b, and PfRh4 recombinant proteins induced antibodies that potently blocked merozoite invasion. This validates the use of a combination of these ligands as a potential vaccine that would have broad activity against P. falciparum. | - |
dc.description.statementofresponsibility | Sash Lopaticki, Alexander G. Maier, Jennifer Thompson, Danny W. Wilson, Wai-Hong Tham, Tony Triglia, Alex Gout, Terence P. Speed, James G. Beeson, Julie Healer, and Alan F. Cowman | - |
dc.language.iso | en | - |
dc.publisher | American Society for Microbiology | - |
dc.relation.isreplacedby | 2440/89928 | - |
dc.relation.isreplacedby | http://hdl.handle.net/2440/89928 | - |
dc.rights | Copyright © 2011, American Society for Microbiology. All Rights Reserved. | - |
dc.source.uri | http://dx.doi.org/10.1128/iai.01021-10 | - |
dc.subject | Erythrocytes | - |
dc.subject | Reticulocytes | - |
dc.subject | Animals | - |
dc.subject | Rabbits | - |
dc.subject | Humans | - |
dc.subject | Plasmodium falciparum | - |
dc.subject | Malaria | - |
dc.subject | Protozoan Proteins | - |
dc.subject | Malaria Vaccines | - |
dc.subject | Antibodies, Protozoan | - |
dc.subject | Immunoblotting | - |
dc.subject | Enzyme-Linked Immunosorbent Assay | - |
dc.subject | Coculture Techniques | - |
dc.subject | Transfection | - |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject | Gene Knockout Techniques | - |
dc.title | Reticulocyte and erythrocyte binding-like proteins function cooperatively in invasion of human erythrocytes by malaria parasites | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1128/IAI.01021-10 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Wilson, D. [0000-0002-5073-1405] | - |
Appears in Collections: | Aurora harvest 7 Microbiology and Immunology publications |
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