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https://hdl.handle.net/2440/9137
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Type: | Journal article |
Title: | Na+-H+ exchange in salivary secretory cells is controlled by an intracellular Na+ receptor |
Author: | Ishibashi, H. Dinudom, A. Harvey, K. Kumar, S. Young, J. Cook, D. |
Citation: | Proceedings of the National Academy of Sciences of USA, 1999; 96(17):9949-9953 |
Publisher: | NATL ACAD SCIENCES |
Issue Date: | 1999 |
ISSN: | 0027-8424 1091-6490 |
Statement of Responsibility: | H. Ishibashi, A. Dinudom, K. F. Harvey, S. Kumar, J. A. Young, and D. I. Cook |
Abstract: | It recently has been shown that epithelial Na(+) channels are controlled by a receptor for intracellular Na(+), a G protein (G(o)), and a ubiquitin-protein ligase (Nedd4). Furthermore, mutations in the epithelial Na(+) channel that underlie the autosomal dominant form of hypertension known as Liddle's syndrome inhibit feedback control of Na(+) channels by intracellular Na(+). Because all epithelia, including those such as secretory epithelia, which do not express Na(+) channels, need to maintain a stable cytosolic Na(+) concentration ([Na(+)](i)) despite fluctuating rates of transepithelial Na(+) transport, these discoveries raise the question of whether other Na(+) transporting systems in epithelia also may be regulated by this feedback pathway. Here we show in mouse mandibular secretory (endpiece) cells that the Na(+)-H(+) exchanger, NHE1, which provides a major pathway for Na(+) transport in salivary secretory cells, is inhibited by raised [Na(+)](i) acting via a Na(+) receptor and G(o). This inhibition involves ubiquitination, but does not involve the ubiquitin protein ligase, Nedd4. We conclude that control of membrane transport systems by intracellular Na(+) receptors may provide a general mechanism for regulating intracellular Na(+) concentration. |
Keywords: | Salivary Glands Animals Mice Sodium GTP-Binding Proteins Ligases Ubiquitin-Protein Ligases Calcium-Binding Proteins Ubiquitins Patch-Clamp Techniques Hydrogen-Ion Concentration Feedback Male Endosomal Sorting Complexes Required for Transport Nedd4 Ubiquitin Protein Ligases Sodium-Hydrogen Exchangers |
DOI: | 10.1073/pnas.96.17.9949 |
Published version: | http://dx.doi.org/10.1073/pnas.96.17.9949 |
Appears in Collections: | Aurora harvest Medicine publications |
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